1. The clinical and phylogenetic investigation for a nosocomial outbreak of respiratory syncytial virus infection in an adult hemato-oncology unit
- Author
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Sawako Nakachi, Sakuko Maeshiro, Jiro Fujita, Toshihiro Horii, Hiroaki Masuzaki, Ayako Uehara, Gretchen Parrott, Takeaki Tomoyose, Saifun Nahar, Masashi Nakamatsu, Takeshi Kinjo, Daisuke Motooka, Masao Tateyama, Shusaku Haranaga, Shota Nakamura, and Daijiro Nabeya
- Subjects
Adult ,Male ,0301 basic medicine ,Time Factors ,Genotype ,Genotyping Techniques ,030106 microbiology ,Respiratory Syncytial Virus Infections ,Polymerase Chain Reaction ,Virus ,Disease Outbreaks ,law.invention ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Japan ,law ,Nasopharynx ,Virology ,Humans ,Medicine ,030212 general & internal medicine ,Viral shedding ,Genotyping ,Polymerase chain reaction ,Aged ,Retrospective Studies ,Cross Infection ,Molecular Epidemiology ,business.industry ,Outbreak ,Middle Aged ,Reverse transcriptase ,Respiratory Syncytial Viruses ,Virus Shedding ,Infectious Diseases ,Real-time polymerase chain reaction ,Rapid antigen test ,Hematologic Neoplasms ,Female ,business - Abstract
Although many reports have already shown RSV outbreaks among hemato-oncology patients, genomic studies detecting similar RSV strains prior to an outbreak in the hospital are rare. In 2014, the University of the Ryukyus hospital hemato-oncology unit experienced, and successfully managed, a respiratory syncytial virus (RSV) nosocomial outbreak. During the outbreak investigation, genotyping and phylogenetic analysis was used to identify a potential source for the outbreak. Nasopharyngeal swabs were tested for RSV using three tests: (1) rapid antigen test (RAT); (2) reverse transcriptase polymerase chain reaction (PCR); or (3) quantitative PCR (RT-qPCR); a positive PCR reaction was considered a confirmed case of RSV. Phylogenetic analysis of the G protein was performed for outbreak and reference samples from non-outbreak periods of the same year. In total, 12 confirmed cases were identified, including 8 hemato-oncology patients. Patient samples were collected weekly, until all confirmed RSV cases returned RSV negative test results. Median time of suspected viral shedding was 16 days (n = 5, range: 8-37 days). Sensitivity and specificity of the RAT compared with RT-qPCR were 30% and 91% (n = 42). Phylogenetic analysis revealed nine genetically identical strains; eight occurring during the outbreak time period and one strain was detected 1 month prior. A genetically similar RSV detected 1 month before is considered one potential source of this outbreak. As such, healthcare providers should always enforce standard precautions, especially in the hemato-oncology unit.
- Published
- 2017