1. Anti-Inflammatory Activity of Epimedium brevicornu Maxim Ethanol Extract
- Author
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Bin Li, Chang Bingquan, Xianghua Quan, Shan Huang, Meng Ning, and Ruiying Yuan
- Subjects
0301 basic medicine ,Male ,Lipopolysaccharide ,medicine.drug_class ,NF-E2-Related Factor 2 ,education ,Anti-Inflammatory Agents ,Medicine (miscellaneous) ,Prostaglandin ,Pharmacology ,Peritonitis ,Nitric Oxide ,Anti-inflammatory ,Dinoprostone ,Nitric oxide ,Proinflammatory cytokine ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,medicine ,Animals ,Humans ,Epimedium ,Nutrition and Dietetics ,biology ,Interleukin-6 ,Plant Extracts ,Macrophages ,Interleukin ,Nitric oxide synthase ,030104 developmental biology ,RAW 264.7 Cells ,chemistry ,030220 oncology & carcinogenesis ,biology.protein ,lipids (amino acids, peptides, and proteins) ,Tumor necrosis factor alpha ,Heme Oxygenase-1 - Abstract
Epimedium brevicornu Maxim has been used as a traditional herbal drug in China. In this study, the anti-inflammatory effects of E. brevicornu Maxim ethanol extract (EBME) were investigated in RAW264.7 macrophages and mice challenged with lipopolysaccharide (LPS). Results showed that EBME attenuated inflammation by decreasing the production of several proinflammatory mediators, such as nitric oxide (NO), prostaglandin (PG) E2, inducible nitric oxide synthase, and cyclooxygenase-2, in LPS-stimulated RAW264.7 macrophages. EBME increased the expression of heme oxygenase-1 (HO-1) and promoted the nuclear translocation of nuclear factor erythroid 2-related factor 2. The inhibitory effects of EBME on LPS-stimulated NO and PGE2 expression were partially reversed by HO-1 inhibitor. EBME also elicited an anti-inflammatory effect by inhibiting the production of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 in LPS-induced peritonitis. Therefore, EBME exhibited anti-inflammatory effects in vitro and ...
- Published
- 2018