1. Influences of Flavonoids on Erythrocyte Membrane and Metabolic Implication Through Anionic Exchange Modulation
- Author
-
Silvana Ficarra, Ester Tellone, Davide Barreca, Ugo Leuzzi, Giuseppina Laganà, Ersilia Bellocco, and Antonio Galtieri
- Subjects
Male ,Antioxidant ,Physiology ,Iron ,medicine.medical_treatment ,Biophysics ,Butylated Hydroxyanisole ,Hemolysis ,Antioxidants ,Band 3 protein ,chemistry.chemical_compound ,Flavonols ,Picrates ,Membrane integrity ,Caspase-3 ,Metabolic modulation ,medicine ,Humans ,Butylated hydroxytoluene ,Hydrogen peroxide ,Flavonoids ,chemistry.chemical_classification ,Molecular Structure ,Autoxidation ,Biphenyl Compounds ,Erythrocyte Membrane ,Hesperetin ,Hydrogen Peroxide ,Cell Biology ,Butylated Hydroxytoluene ,Ascorbic acid ,Kinetics ,chemistry ,Biochemistry ,Butylated hydroxyanisole ,Oxidation-Reduction - Abstract
The antioxidative activity of some natural flavonoids was analyzed against the stable free radical 2,2-diphenyl-1-picryhydrazyl. The results indicate that the scavenging power of the tested flavonols is higher than that of the synthetic antioxidants butylated hydroxyanisole and butylated hydroxytoluene; instead, the flavanones show little activity, as indicated by efficient concentration (EC50) values. Flavonoid autoxidation and interaction with Fe2+ and hydrogen peroxide were tested using erythrocyte membranes as a model. The results show that some compounds, like hesperetin, evidence a pro-oxidant activity higher than the ascorbic acid/iron reference system. The compounds with strong oxidative capability do not only influence cellular redox balance but also activate caspase-3, producing lactate dehydrogenase release and enhancing anionic exchange at the level of band 3 protein.
- Published
- 2009