1. Genetic diversity of Clavispora lusitaniae isolated from Agave fourcroydes Lem, as revealed by DNA fingerprinting
- Author
-
Alberto Cortes-Velazquez, Daisy Pérez-Brito, Teófilo Herrera-Suarez, Claudia Torres-Calzada, Alfonso Larqué-Saavedra, Raul Tapia-Tussell, Anuar Magaña-Álvarez, and Patricia Lappe-Oliveras
- Subjects
Mycobiota ,DNA, Ribosomal ,Polymerase Chain Reaction ,Applied Microbiology and Biotechnology ,Microbiology ,Agave ,Clavispora lusitaniae ,Polymorphism (computer science) ,DNA, Fungal ,Structural motif ,Mexico ,Ribosomal DNA ,Phylogeny ,Genetics ,Genetic diversity ,Polymorphism, Genetic ,biology ,Alcoholic Beverages ,Microbiota ,Genetic Variation ,Sequence Analysis, DNA ,General Medicine ,Agave fourcroydes ,biology.organism_classification ,DNA Fingerprinting ,DNA profiling ,Fermentation ,Saccharomycetales - Abstract
This study characterized Clavispora lusitaniae strains isolated from different stages of the processing and early fermentation of a henequen (Agave fourcroydes) spirit produced in Yucatan, Mexico using a molecular technique. Sixteen strains identified based on morphological features, obtained from different substrates, were typed molecularly. Nine different versions of the divergent D1/D2 domain of the large-subunit ribosomal DNA sequence were identified among the C. lusitaniae strains. The greatest degree of polymorphism was found in the 90-bp structural motif of the D2 domain. The MSP-PCR technique was able to differentiate 100% of the isolates. This study provides significant insight into the genetic diversity of the mycobiota present during the henequen fermentation process, especially that of C. lusitaniae, for which only a few studies in plants have been published. The applied MSP-PCR markers were very efficient in revealing olymorphisms between isolates of this species.
- Published
- 2015
- Full Text
- View/download PDF