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Start Over Author "Caggese, C." Journal journal of molecular biology
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2. Homologous nuclear genes encode cytoplasmic and mitochondrial glutamine synthetase in Drosophila melanogaster

Author

Corrado Caggese, F. Ritossa, Ruggiero Caizzi, Maria Pia Bozzetti, Caizzi, R., Bozzetti, Maria Giuseppina, Caggese, C., and Ritossa, F.

Subjects
molecular cloning, Gene isoform, Cytoplasm, Nuclear gene, Molecular Sequence Data, Gene Expression, Homology (biology), Structural Biology, Glutamate-Ammonia Ligase, Drosophilidae, Glutamine synthetase, Sequence Homology, Nucleic Acid, Melanogaster, Animals, Humans, Amino Acid Sequence, Cloning, Molecular, Molecular Biology, Gene, isozyme, Genetics, Genomic Library, biology, Base Sequence, Nucleic Acid Hybridization, biology.organism_classification, Mitochondria, mitochondria, Isoenzymes, Drosophila melanogaster, Biochemistry, Nucleic Acid Conformation
Abstract

We describe the cloning of the glutamine synthetase 1 (GS1) gene based on cross-homology with the glutamine synthetase 2 (GS2) gene in Drosophila melanogaster. We have determined the GS gene number in the Drosophila genome, and we describe the isolation of cDNA clones corresponding to the two isoforms, their entire sequence and their transcription pattern. We looked for subcellular localization of one enzymic isoform; in this way, we were able to locate the GS1 enzyme within the mitochondria of D. melanogaster. We have compared different GS sequences from plants and humans; emerging evolutionary implications are discussed. In addition, we have identified a certain highly stable secondary structure at the nucleotide level in the coding region of isoforms located in the organella.

Published
1990

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