1. Expert system for protein engineering: its application in the study of chloramphenicol acetyltransferase and avian pancreatic polypeptide
- Author
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Alan Marsden, Eric Platt, Robert V. Fishleigh, Barry Robson, and Peter Millard
- Subjects
Chloramphenicol acetyltransferase ,Protein structure ,Biochemistry ,De novo protein structure prediction ,Acetyltransferase ,Biophysics ,Pancreatic polypeptide ,Protein engineering ,Biology ,Protein secondary structure ,Homology (biology) - Abstract
Lucifer is a suite of programs for the conformational study of drugs, proteins and other biomolecules. The overall architecture and operation of the suite is outlined with worked examples. New procedures are also described for the identification of secondary structure template similarity based on theorem-proof algebra and for the rapid evaluation of the hydrophobic packing of a protein conformation. These are discussed in relation to the molecular-graphics modelling of chloramphenicol acetyltransferase. Although Lucifer is of proven worth for the study of biological peptides and for protein modelling against homologues, its applicability to de novo protein structure prediction is untested. A preliminary study of avian pancreatic polypeptide is of this character and is described here. The results of the above attempts are both informative and promising.
- Published
- 1987
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