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The alpine treeline ecotone is an important component of mountain ecosystems of the Nepal Himalaya; it plays a vital role in the livelihood of indigenous people, and provides ecosystem services. However, the region faces a problem of paucity of data on treeline characteristics at the regional and landscape scales. Therefore, we used Remote Sensing (RS), and Geographic Information Science (GIS) approaches to investigate cross-scale interactions in the treeline ecotone. Additionally, European Space Agency land cover map, International Center for Integrated Mountain Development (ICIMOD) land cover map, ecological map of Nepal, and United States Geological Survey Shuttle Radar Topography Mission-Digital Elevation Model were used to analyze treeline pattern at the regional scale. Digital Globe high-resolution satellite imagery of Barun (eastern Nepal) and Manang (central Nepal) were used to study treeline patterns at the landscape scale. Treeline elevation ranges from 3300-4300 m above sea level. Abies spectabilis, Betula utilis, and Pinus wallichiana are the main treeline-forming species in the Nepal Himalaya. There is an east to west treeline elevation gradient at the regional scale. No slope exposure is observed at the regional scale; however, at the landscape scale, slope exposure is present only in a disturbed area (Manang). Topography and human disturbance are the main treeline controlling factor in Barun and Manang respectively.

Treeline ecotone dynamics of Abies spectabilis(D. Don) Mirb.in the Barun valley, Makalu Barun National Park, eastern Nepal Himalaya were studied by establishing seven plots (20 m × variable length) from the forestline to the tree species limit: three plots on the south- and north-facing slopes each (S1-S3, N1-N3), and one plot on the east-facing slope (E) in the relatively undisturbed forests. A dendroecological method was used to study treeline advance rate and recruitment pattern. In all the plots, most trees established in the early 20th century, and establishment in the second half of the 20th century was confined to the forestline area. Treeline position has not advanced substantially in the Barun valley, with only 22 m average elevational shift in the last 130 years, and with average current shifting rate of 14 cm/yr. Moreover, no significant relationship was found between tree age and elevation on the south-, north-, and east-facing slopes. The number of seedlings and saplings in near the treeline area was negligible compared to that near the forestline area. Therefore, A. spectabilis treeline response to the temperature change was slow, despite the increasing temperature trend in the region. Beside the temperature change, factors such as high inter-annual variability in temperature, dense shrub cover, and local topography also play an important role in treeline advance and controlling recruitment pattern above the treeline.