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[Objectives]The objective of this study was to get deeper insight in the effect of fenarimol application on Magnaporthe poae and fungi properties in the M.poae infected soil. [Methods]Based on the 18s rRNA internal transcribed spacer(ITS)gene,two metagenomic libraries from control and fenarimol treated rhizosphere soil were constructed,and deeper sequencing was carried out by Illumina Genome Analyzer(GA)technology. [Results]There were 4 851 438 and 3 037 924 Illumina reads generated from control and fenarimol treated rhizosphere soil samples,which were further clusterd to 28 219 and 21 288 repetitive operational taxonomic unit(OTU). After fenarimol treated,Ascomycota decreased 18% compared with the control, while Basidiomycota increased 32%,and there were no significant changes of Chytridiomycota and Glomeromycot. In addition,there were 7 phyla,152 genera and 200 fungi species that disappeared after fungicide application. [Conclusions]Fenarimol had significant suppression on M.poae and Phoma, Leptosphaeria, Fusariuma, Phialophora communities in rhizosphere soil. [ABSTRACT FROM AUTHOR]

Summer patch is a destructive disease of Kentucky bluegrass (Poa pratensis) caused by Magnaporthe poae, which usually begins to emerge when the soil temperature and humidity are high. In this study, we sequenced 24 Magnaporthe and its related strains based on the ITS1-5.8S-ITS2 gene, and then investigated the ITS phylogennetic characterization. The results suggested that stains of M.poae and its related species distributed to two phylogennetic groups(A and B). M. oryzae and Pyricularia oryzae formed group A and was further divided into two clades, suggesting that M. oryzae is more closely related to P. oryzae, these species infect both root and aerial parts of the plant host. Group B contains M. salvinii, M. poae, M. rhizophila, Gaeumannomyces incrustans, G. graminis var. graminis and G. graminis var. tritici. These species of group B could infect root aparts of the plant host when excluding M. salvinii. In the turf environment, the detection of the species of group B is more difficult than that of group A if by morphological detection. Based on the molecular characterization of ITS gene, we produced a macroarray, which included three types of oligonucleotide probes: monomers (20-24 bp), dimers (40-48 bp), and monomers with a poly-A tail of 20 (30-44 bp) bases, respectively. The results showed that M. poae, G. incrustans and G. graminis of group B could be quickly detected and identified by the designed array. The detect sensitivities of dimers are significantly higher than the monomers-A20, still higher than monomers. The use of repeat sequence probes (dimers) greatly improved the sensitivity of the macroarray, which can be used in turf pathogen detection in the future. [ABSTRACT FROM AUTHOR]