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Lateral migrations are movements of fish schools to and from lentic and/or lotic environments, in order to feed, reproduce or seek favorable survival conditions. This study was conducted in the corixo Manoel Isaac, which connects Cuiabá River to Chacororé Lake in the Pantanal of Barão of Melgaço, State of Mato Grosso. Fish were collected in the period of April-December, that included the seasons of receding water, drought and flood aiming to analyze the lateral migration of fish. It was observed that 12 species used the systems during dry and flood seasons, to feed (time of "lufada") and to spawn (time of "spawning"), respectively. When the water level is higher, the fish migrate from the bay to the river. At the beginning of the ebb (April), the catch is high, extending up to July, and CPUEs decrease when the drought period begins (July) and there is a rise in the water level of the corixo at the end of September. A second elevation in the catch is verified; however, the direction of movement of the majority of the species is from the river to the bay, following water current. It was concluded that the lateral migration of fish between the Chacororé Bay and the Cuiabá River during the study presented greater catches during the months of April to June (ebb) with fish moving from the bay to the river and during the flood (October to December), the fish migrate in the opposite, from the river towards bay. [ABSTRACT FROM AUTHOR]