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Your search keyword '"S, Bellafqih"' showing total 3 results
Start Over Author "S, Bellafqih" Journal journal of neuroendocrinology
3 results on '"S, Bellafqih"'

1. Biochemical Characterisation and Immunohistochemical Localisation of the Secretogranin II-Derived Peptide EM66 in the Hypothalamus of the Jerboa (Jaculus orientalis): Modulation by Food Deprivation

Author

S. Bellafqih, M. Boutahricht, Hubert Vaudry, Rabia Magoul, A. Alaoui, S. El Ouezzani, Laurent Yon, Maité Montero-Hadjadje, Johann Guillemot, and Youssef Anouar

Subjects
medicine.medical_specialty, Secretoneurin, Lateral hypothalamus, Endocrine and Autonomic Systems, Adrenal gland, Endocrinology, Diabetes and Metabolism, Neuropeptide, 030209 endocrinology & metabolism, Biology, Preoptic area, 03 medical and health sciences, Cellular and Molecular Neuroscience, 0302 clinical medicine, Endocrinology, medicine.anatomical_structure, nervous system, Arcuate nucleus, Hypothalamus, Parvocellular cell, Internal medicine, medicine, 030217 neurology & neurosurgery
Abstract

The neuroendocrine protein secretogranin II is the precursor of several neuropeptides, including secretoneurin and a novel 66-amino acid peptide, EM66, the sequence of which has been highly conserved across the vertebrae phylum. The presence of EM66 has been detected in the adult and fetal human adrenal gland, as well as the rat pituitary and adrenal glands. The present study aimed to explore a possible neuroendocrine role of EM66 by analysing its occurrence and distribution within the jerboa hypothalamus, and its potential implication in the control of feeding behaviour. High-performance liquid chromatography analysis of jerboa hypothalamic extracts combined with a radioimmunoassay of EM66 revealed a single peak of immunoreactive material exhibiting the same retention time as recombinant EM66. Immuno-cytochemical labelling showed that EM66-producing neurones are widely distributed in several hypothalamic regions, including the preoptic area, the suprachiasmatic, supraoptic, parvocellular paraventricular and arcuate nuclei, and the lateral hypothalamus. Food deprivation for 5 days induced a significant increase in the number of EM66-containing neurones within the arcuate nucleus (105% increase) and the parvocellular aspect of the paraventricular nucleus (115% increase), suggesting that EM66 could be involved in the control of feeding behaviour and/or the response to stress associated with fasting. Altogether, these data reveal the physiological plasticity of the EM66 system in the hypothalamus and implicate this novel peptide in the regulation of neuroendocrine functions.

Published
2005

2. Biochemical characterisation and immunohistochemical localisation of the secretogranin II-derived peptide EM66 in the hypothalamus of the jerboa (Jaculus orientalis): modulation by food deprivation

Author

M, Boutahricht, J, Guillemot, M, Montero-Hadjadje, S, Bellafqih, S, El Ouezzani, A, Alaoui, L, Yon, H, Vaudry, Y, Anouar, and R, Magoul

Subjects
Male, Molecular Sequence Data, Arcuate Nucleus of Hypothalamus, Rodentia, Feeding Behavior, Immunohistochemistry, Peptide Fragments, Secretogranin II, Chromogranins, Animals, Female, Amino Acid Sequence, Food Deprivation, Paraventricular Hypothalamic Nucleus
Abstract

The neuroendocrine protein secretogranin II is the precursor of several neuropeptides, including secretoneurin and a novel 66-amino acid peptide, EM66, the sequence of which has been highly conserved across the vertebrae phylum. The presence of EM66 has been detected in the adult and fetal human adrenal gland, as well as the rat pituitary and adrenal glands. The present study aimed to explore a possible neuroendocrine role of EM66 by analysing its occurrence and distribution within the jerboa hypothalamus, and its potential implication in the control of feeding behaviour. High-performance liquid chromatography analysis of jerboa hypothalamic extracts combined with a radioimmunoassay of EM66 revealed a single peak of immunoreactive material exhibiting the same retention time as recombinant EM66. Immunocytochemical labelling showed that EM66-producing neurones are widely distributed in several hypothalamic regions, including the preoptic area, the suprachiasmatic, supraoptic, parvocellular paraventricular and arcuate nuclei, and the lateral hypothalamus. Food deprivation for 5 days induced a significant increase in the number of EM66-containing neurones within the arcuate nucleus (105% increase) and the parvocellular aspect of the paraventricular nucleus (115% increase), suggesting that EM66 could be involved in the control of feeding behaviour and/or the response to stress associated with fasting. Altogether, these data reveal the physiological plasticity of the EM66 system in the hypothalamus and implicate this novel peptide in the regulation of neuroendocrine functions.

Published
2005

3. Biochemical characterisation and immunohistochemical localisation of the secretogranin II-derived peptide EM66 in the hypothalamus of the jerboa (Jaculus orientalis): modulation by food deprivation.

Author

Boutahricht M, Guillemot J, Montero-Hadjadje M, Bellafqih S, El Ouezzani S, Alaoui A, Yon L, Vaudry H, Anouar Y, and Magoul R

Subjects
Amino Acid Sequence, Animals, Chromogranins chemistry, Feeding Behavior physiology, Female, Immunohistochemistry, Male, Molecular Sequence Data, Peptide Fragments chemistry, Rodentia, Secretogranin II chemistry, Arcuate Nucleus of Hypothalamus metabolism, Chromogranins metabolism, Food Deprivation physiology, Paraventricular Hypothalamic Nucleus metabolism, Peptide Fragments metabolism, Secretogranin II metabolism
Abstract

The neuroendocrine protein secretogranin II is the precursor of several neuropeptides, including secretoneurin and a novel 66-amino acid peptide, EM66, the sequence of which has been highly conserved across the vertebrae phylum. The presence of EM66 has been detected in the adult and fetal human adrenal gland, as well as the rat pituitary and adrenal glands. The present study aimed to explore a possible neuroendocrine role of EM66 by analysing its occurrence and distribution within the jerboa hypothalamus, and its potential implication in the control of feeding behaviour. High-performance liquid chromatography analysis of jerboa hypothalamic extracts combined with a radioimmunoassay of EM66 revealed a single peak of immunoreactive material exhibiting the same retention time as recombinant EM66. Immunocytochemical labelling showed that EM66-producing neurones are widely distributed in several hypothalamic regions, including the preoptic area, the suprachiasmatic, supraoptic, parvocellular paraventricular and arcuate nuclei, and the lateral hypothalamus. Food deprivation for 5 days induced a significant increase in the number of EM66-containing neurones within the arcuate nucleus (105% increase) and the parvocellular aspect of the paraventricular nucleus (115% increase), suggesting that EM66 could be involved in the control of feeding behaviour and/or the response to stress associated with fasting. Altogether, these data reveal the physiological plasticity of the EM66 system in the hypothalamus and implicate this novel peptide in the regulation of neuroendocrine functions.

Published
2005
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