1. Peroxisome proliferator-activated receptor-γ agonists suppress iNOS expression induced by LPS in rat primary Schwann cells
- Author
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Zhang, Fupeng, Liu, Fen, Yan, Meijuan, Ji, Huoyan, Hu, Ling, Li, Xiaohong, Qian, Ji, He, Xingxin, Zhang, Li, Shen, Aiguo, and Cheng, Chun
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NUCLEAR receptors (Biochemistry) , *ENDOTOXINS , *NEURITIS , *GENE expression , *MYELIN sheath , *NITRIC-oxide synthases , *LABORATORY rats , *MULTIPLE sclerosis - Abstract
Abstract: In bacterial-induced peripheral nervous system (PNS) inflammation, Schwann cells (SCs) are activated, producing inducible nitric oxide synthase (iNOS), contributed to the pathogenesis of demyelinating disease, such as multiple sclerosis. Peroxisome proliferator-activated receptor-gamma (PPAR-γ) has been shown to play a protective role in cellular inflammatory responses. Here we showed that LPS-induced iNOS biosynthesis was in a concentration and time-dependent manner. In LPS-treated primary SCs, retreatment with PPAR-γ agonist remitted the increase of iNOS, p38 phosphorylation and TLR4, MyD88, augmented the expression of PPAR-γ and localization in nuclear. Coadministration of GW 9662 reversed the effect of PPAR-γ agonists. These results suggest that PPAR-γ agonists, 15d-PGJ2 and pioglitazone, had the anti-inflammatory effects. [Copyright &y& Elsevier]
- Published
- 2010
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