1. Diethylene glycol distearate (DGD): a versatile embedding medium for retinal cytochemistry
- Author
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Jun C. Huang, Kristina Mieziewska, Theo van Veen, Gustavo D. Aguirre, and Nancy Philp
- Subjects
Transcription, Genetic ,Diethylene glycol distearate ,Fluorescent Antibody Technique ,Retina ,Embedding Medium ,chemistry.chemical_compound ,Dogs ,Retinal Rod Photoreceptor Cells ,Lectins ,medicine ,Animals ,RNA, Messenger ,In Situ Hybridization ,Chemistry ,General Neuroscience ,Histological Techniques ,Rod Opsins ,Retinal ,Haplorhini ,RNA Probes ,Immunohistochemistry ,medicine.anatomical_structure ,Antisense Elements (Genetics) ,Biochemistry ,Cytochemistry ,Retinal Cone Photoreceptor Cells ,Ethylene Glycols - Abstract
Embedment in diethylene glycol distearate (DGD) was shown to be highly desirable and versatile for retinal cytochemical studies, including in situ hybridization, immuno- and lectin cytochemistry. This method allows for preservation of fine tissue detail as well as good reaction sensitivity. It appears to be more suitable than most other methods currently used for light microscopic retinal cytochemistry.
- Published
- 1993