1. Construction of Plant Expression Vector for Hand, Foot and Mouth Virus EV71-VP1 Gene and Its Expression in Tomato.
- Author
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Wang Mei-liang, Zhao Yue, Wang Yu-dan, Li Xin-zhi, Zhang Yao, Chen Xiu-ling, Qiu You-wen, and Wang Ao-xue
- Subjects
GENE expression in plants ,TOMATOES ,FOOT & mouth disease ,FOOT & mouth disease virus ,GENE fusion ,REVERSE transcriptase polymerase chain reaction ,GENETIC vectors - Abstract
EV71-type virus is one of the main pathogens causing the occurrence of hand, foot and mouth disease (HFMD), and VP1 protein, a factor that directly determines the antigenicity of the virus, has been isolated. The tomato was selected as a bioreactor for the production of an edible EV71 vaccine designed for the VP1 capsid protein. Using molecular biology techniques, the fusion gene EV71-VP1 was cut from vector PGEX-4T-2, a vector containing the p2300-EV71 gene with CaMV35S promoter and TL regulatory elements was constructed, and the hypocotyl and cotyledons of tomato were transformed using Agrobacterium (EHA105)-mediated method, screened, elongated and rooted, and finally 20 resistant tomato plants were obtained. Five transgenic positive seedlings were obtained by digestion and PCR assay, among which three plants were detected by RT-PCR to be capable of transcriptional translation at the RNA level. The experimental results aimed to explore new material support for the preparation of transgenic plant oral vaccines against EV71 infection and provide a theoretical basis for accelerating the development of transgenic plant vaccines in the future. [ABSTRACT FROM AUTHOR]
- Published
- 2023