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Medicinal fungi refers to large fungi that are widely used in the field of traditional Chinese medicine and have certain therapeutic effects. Increasing and improving the yield and quality of medicinal fungi is an important goal of medicinal fungi breeding,and compared with other mutagenesis methods,physical mutagenesis has played an important role in the field of medicinal fungi mutagenesis breeding. This article reviews 9 different physical mutagenesis breeding methods including ultraviolet mutagenesis,ionizing radiation mutagenesis,laser mutagenesis,ion beam mutagenesis,ultrasonic mutagenesis,plasma mutagenesis,neutron mutagenesis,space mutagenesis,and compound mutagenesis and the research progress of physical mutagenesis technology in the field of medicinal fungi,and prospects for its application. [ABSTRACT FROM AUTHOR]

【Objective】 To provide reference for the studies on cell mutagenesis, fusion and gene editing of Hypsizygus marmoreus.【Methods】 The single factor test and response surface method were used to screen and optimize the main factors affecting the preparation and regeneration conditions of the protoplasts of Hypsizygus marmoreus—bacterial age, osmotic pressure stabilizer types, lysozyme concentration and enzymolysis time.【Results】 The single factor test results showed that the optimal preparation condition of protoplasts were 7 days of bacterial age, with the concentration of 0.6 mol/L mannitol as osmotic pressure stabilizer, the lysozyme concentration of 2%, enzymolysis for 5 h. The optimal regeneration conditions of protoplasts were 6 days of bacterial age, with the concentration of 0.6 mol/L mannitol as osmotic pressure stabilizer, the lysozyme concentration of 2%, enzymolysis for 3 h. The response surface method showed that when 0.6 mol/L mannitol was used as the osmotic pressure stabilizer, the best preparation and regeneration conditions were 7 days of bacterial age, 2% of lysozyme concentration, and 4 h of enzymolysis, which had good fitting with the predicted value of the model.【Conclusion】 Based on the optimal preparation conditions determined by response surface method, higher protoplast yield and regeneration rate could be obtained. [ABSTRACT FROM AUTHOR]