1. Upregulation of Heme Oxygenase-1 Expression in Gingiva After Cyclosporin A Treatment
- Author
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Hsien-Chung Chiu, Yu-Tang Chin, Nieh Shin, Hsiao-Pei Tu, Guo-Lun Lan, Earl Fu, Martin M. Fu, and Cheng-Yang Chiang
- Subjects
Male ,medicine.medical_specialty ,Gingiva ,Rats, Sprague-Dawley ,Random Allocation ,chemistry.chemical_compound ,Downregulation and upregulation ,In vivo ,Internal medicine ,Cyclosporin a ,medicine ,Animals ,Humans ,RNA, Messenger ,Heme ,Cells, Cultured ,business.industry ,Fibroblasts ,Cytoprotection ,Rats ,Up-Regulation ,Blot ,Heme oxygenase ,Endocrinology ,chemistry ,Gingival Hyperplasia ,Immunology ,Cyclosporine ,Periodontics ,Immunohistochemistry ,business ,Heme Oxygenase-1 ,Immunosuppressive Agents - Abstract
Heme oxygenase (HO)-1 is a stress-inducible protein that confers cytoprotection, but its role in gingiva during cyclosporin A (CsA) therapy is unknown. We used in vivo and in vitro models to investigate the expression of mRNA and protein for HO-1 in gingiva upon CsA treatment.Twenty-six male Sprague-Dawley rats were assigned to two groups after the establishment of edentulous ridges. Rats in the CsA group received CsA, 30 mg/kg/day for 4 weeks, whereas control rats received mineral oil only. All rats were killed after 4 weeks, and the edentulous gingivae were excised. mRNA and protein expression of HO-1 in gingivae were determined using reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC), respectively. For the in vitro study, cultured human gingival fibroblasts were harvested after treatment with various concentrations of CsA, and HO-1 mRNA and protein expression were determined using RT-PCR and Western blotting, respectively.Mean gingival HO-1 mRNA expression was greater in the CsA group than in the control animals (P = 0.076). IHC staining for HO-1 protein was significantly greater in the gingivae of CsA-treated rats than in those of the control group. In fibroblast cultures, expression of HO-1 mRNA and protein also increased significantly after CsA treatment.CsA upregulates the gingival expression of HO-1, which may exert a cytoprotective effect.
- Published
- 2008