1. A validated UHPLC-MS/MS method for pharmacokinetic and brain distribution studies of twenty constituents in rat after oral administration of Jia-Wei-Qi-Fu-Yin.
- Author
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An, Hai-Ming, Li, Meng-Ning, Yang, Hua, Pang, Han-Qing, Qu, Cheng, Xu, Yi, Liu, Run-Zhou, Peng, Chao, Li, Ping, and Gao, Wen
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FERULIC acid , *SAPONINS , *TANDEM mass spectrometry , *PHARMACOKINETICS , *PSEUDOPOTENTIAL method , *RATS , *PHENOLIC acids - Abstract
• A rapid, sensitive and selective UHPLC-MS/MS method was developed and validated. • Twenty components were quantified in rat plasma and brain orally administrated by JWQFY. • These results were further applied to pharmacokinetic and brain distribution studies. • Six compounds were considered as the potential effective components of JWQFY. A rapid ultra-high performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry (UHPLC-QqQ MS/MS) approach with high sensitivity and selectivity was developed for the quantification of twenty compounds, including 9 saponins, 8 flavonoids, 2 oligosaccharide esters and 1 phenolic acid, in rat plasma and brain, which was administrated intragastrically with Jia-Wei-Qi-Fu-Yin (JWQFY), Mass spectrometric detection was operated under multiple reaction monitoring (MRM) mode. All calibration curves possessed good linearity with correlation coefficients (r2) higher than 0.9916 in their respective linear ranges. For intra- and inter-day precision, all the relative standard deviations (RSDs) at different levels were less than 14.68 %. Based on the UHPLC-QqQ MS/MS quantitative results, pharmacokinetic study and brain distribution of multiple components in JWQFY was then successfully performed. As a result, constituents with discrepancy structures showed diverse pharmacokinetic and distribution characteristics. For instance, ferulic acid (phenolic acid), 3, 6′-disinapoyl sucrose and tenuifoliside A (oligosaccharide esters) showed short T max (< 10 min), whereas the T max of ginsenosides Rb 1 , Rb 2 and Rc (ppd-type terpenoid saponins) were much longer (> 4 h). Besides, ferulic acid, epimedin C, icariin, glycyrrhizin, ginsenoside Rb 1 and ginsenoside Rg 1 were considered as the potential effective ingredients of JWQFY because of their relatively high exposure to blood and brain. Our study would provide relevant information for discovery of pharmacodynamic ingredients, as well as further action mechanisms investigations of JWQFY. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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