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Start Over Descriptor "Release test" Journal journal of pharmaceutical analysis
2 results on '"Release test"'

1. Release test of alliin/alliinase double-layer tablet by HPLC—Allicin determination.

Author

Liang, Yan, Zhang, Jing-Jing, Zhang, Qi-Bing, Wang, Zhong-Xia, Yin, Zong-Ning, Li, Xin-Xia, Chen, Jian, and Ye, Li-Ming

Subjects
ALLIIN, DRUG tablets, HIGH performance liquid chromatography, PHARMACOPOEIAS, CARBOXYLIC acids, HOMOGENEITY
Abstract

Abstract: A simple, precise and accurate method was developed and validated for the determination of allicin release from alliin/alliinase double-layer tablets. According to Appendix XC II of Chinese Pharmacopoeia 2010 edition Volume II, a small glass-method was adopted at the rotational speed of 100r/min using 100mL phosphate buffer (pH 6.8) as release medium. The release amount was determined by HPLC with a C18 column (250mm×4.6mm, 5μm) using the mobile phase consisting of methanol −0.4% carboxylic acid (65:35) at a flow rate of 1mL/min and UV detection at 242nm. The current method demonstrates good linearity over the range 4.052–405.2μg/mL (r 2=0.9999) with an average recovery of 105.5%(RSD=1.25%). The accumulative release of alliin/alliinase double-layer tablets had good homogeneity for within- and between-batches. The method established is simple, accurate and repeatable for the determination of allicin release from alliin/alliinase double-layer tablets. [Copyright &y& Elsevier]

Published
2013
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2. Release test of alliin/alliinase double-layer tablet by HPLC—Allicin determination

Author

Zong-Ning Yin, Xin-Xia Li, Jian Chen, Qi-Bing Zhang, Jing-Jing Zhang, Li-Ming Ye, Yan Liang, and Zhong-Xia Wang

Subjects
Double-layer tablet, Carboxylic acid, Pharmaceutical Science, Pharmacy, Alliin, High-performance liquid chromatography, Article, Analytical Chemistry, chemistry.chemical_compound, Alliinase, Drug Discovery, Electrochemistry, Chinese pharmacopoeia, Spectroscopy, Medicine(all), chemistry.chemical_classification, Chromatography, Allicin, biology, Biochemistry, Genetics and Molecular Biology(all), lcsh:RM1-950, Phosphate buffered saline, lcsh:Therapeutics. Pharmacology, chemistry, Release test, biology.protein, Methanol, HPLC, Alliin/alliinase
Abstract

A simple, precise and accurate method was developed and validated for the determination of allicin release from alliin/alliinase double-layer tablets. According to Appendix XC II of Chinese Pharmacopoeia 2010 edition Volume II, a small glass-method was adopted at the rotational speed of 100r/min using 100mL phosphate buffer (pH 6.8) as release medium. The release amount was determined by HPLC with a C18 column (250mmÃ4.6mm, 5μm) using the mobile phase consisting of methanol â0.4% carboxylic acid (65:35) at a flow rate of 1mL/min and UV detection at 242nm. The current method demonstrates good linearity over the range 4.052â405.2μg/mL (r2=0.9999) with an average recovery of 105.5%(RSD=1.25%). The accumulative release of alliin/alliinase double-layer tablets had good homogeneity for within- and between-batches. The method established is simple, accurate and repeatable for the determination of allicin release from alliin/alliinase double-layer tablets. Keywords: Alliin/alliinase, Double-layer tablet, Allicin, Release test, HPLC

Published
2012

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