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Your search keyword '"Philippe Grosseau"' showing total 2 results
Start Over Author "Philippe Grosseau" Journal journal of physics: conference series
2 results on '"Philippe Grosseau"'

1. Development of innovative pH sensor to evaluate phagocytosis of nanoparticles

Author

Delphine Boudard, Philippe Grosseau, Cédric Louis, Sabine Palle, Jérémie Pourchez, D. Bernache, Laurence Marmuse, Lara Leclerc, and Michèle Cottier

Subjects
Ph probe, History, Chromatography, Chemistry, Phagocytosis, Nanoparticle, Macrophage cell, Fluorescence, Computer Science Applications, Education, Red fluorescence
Abstract

The aim of this work was the development of pH-sensor-NP allowing the quantification of the amount of NP phagocytosed by macrophages. Two types of fluorescent NP with variable and well-characterized sizes and chemicals coatings have been synthesized: NP with a FITC core (FITC-NP): green fluorescence (control). FITC-NP functionalized with a pH sensitive probe (pH-sensor-NP): green fluorescence of the FITC and red fluorescence of the pH probe in acidic conditions. Our pH-sensor-NP model was first validated in acellular conditions. They were then incubated with a macrophage cell line allowing distinction and quantification of internalized NP with no major effects on biological toxicity.

Published
2011
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2. Study on the toxicity of inhaled alumina nanoparticles: impact of physicochemical properties and adsorption artifacts on the measurement of biological responses

Author

Michèle Cottier, Delphine Boudard, Philippe Grosseau, Mélanie Pailleux, Jérémie Pourchez, Laboratoire des Procédés en Milieux Granulaires (LPMG-EMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS), Centre Sciences des Processus Industriels et Naturels (SPIN-ENSMSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Département Poudres et Matériaux Multi-Composants (P2MC-ENSMSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-SPIN, Laboratoire Interdisciplinaire des Nanoparticules Aérosolisées (LINA-ENSME), Institut Fédératif de Recherche en Sciences et Ingénierie de la Santé (IFRESIS-ENSMSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-IFR143, Centre Ingénierie et Santé (CIS-ENSMSE), Laboratoire Interdisciplinaire d'Etude des Nanoparticules Aérosolisées (LINA-ENSMSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-CIS, Service d'Histologie Embryologie, CHRU Saint-Etienne, CHU de Saint Etienne, Université Jean Monnet, and PRES de Lyon

Subjects
chemistry.chemical_classification, inhalation, History, Toxicity, Photon Correlation Spectroscopy, Biomolecule, Nanoparticle, Nanotechnology, Engineered nanoparticles, ultrafine alumina nanoparticles, lung, Computer Science Applications, Education, Adsorption, specific surface area technique, Dynamic light scattering, chemistry, BET method, [SDV.TOX]Life Sciences [q-bio]/Toxicology, laser particles size analyser, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering
Abstract

International audience; This work aims at developping a multidisciplinary approach to highlight the correlation between the toxicity of alumina engineered nanoparticles (NP) and their physicochemical characteristics. Accuracy of measurements depends on cell production after contact with particles, but also depends on the ability of biomolecules to get adsorbed on the NP [1]. That's why, mechanisms of biomolecules adsorption on NP must be fully understood to avoid misinterpretation of data.

Published
2011
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