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Essential oils obtained from Origanumspecies are characterized by high diversity in composition. In this work, thin-layer chromatography (TLC)—fingerprinting of Origanumspecies was performed for the first time and enabled the discrimination of three chemotypes (carvacrol, caryophyllene oxide, and terpineol/ sabinyl). Gas chromatography—mass spectrometry and statistical analyses confirmed the presence of the mentioned chemotypes and deepened the characterization of the studied essential oils. TLC—bioautography showed that thymol and carvacrol are the main antioxidant compounds in Origanumsp. essential oils (EOs).

Plants of the Malvaceaefamily are very rich in phenolic compounds. TLC, 2D TLC, and RP HPLC have been used for qualitative analysis of phenolic acids and flavonoids in the flowers of Lavatera trimestrisL. Four aglycones, seven glycosides and eleven phenolic acids were identified in the plant, most for the first time. Quantitative analysis of phenolic compounds was also performed.

The objective of the work described in this paper was to investigate the separation of ginsenosides by TLC, HPTLC, and OPLC. The best separation of the ginsenosides was obtained by use of OPLC on silica gel 60F254with chloroform—methanol—ethyl acetate—water—hexane, 20 + 22 + 60 + 8 + 4 (v/v), as mobile phase. Results from TLC, HPTLC, and OPLC analysis of ginsenosides indicated that use of the different methods of development resulted in significant differences between the resolution of the compounds. Forced-flow OPLC is the most sensitive method for analysis of the ginsenosides.

Conditions have been established for separation of eight ginsenosides by HPTLC with automated multiple development. The ginsenosides were separated on silica gel 60F254 with methanol-chloro-form mobile phases. Two gradiënt programs and two different values of the increment in the development distance were compared. Factors affecting extraction of the ginsenosides from leaves were investigated. The results show that AMD is suitable for screening of ginsenosides in extracts from ginseng.