1. Cytosolic LOX overexpression in Arabidopsis enhances the attractiveness of parasitic wasps in response to herbivory and incidences of parasitism.
- Author
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Ozawa, Rika, Shiojiri, Kaori, Kishimoto, Kyutaro, Matsui, Kenji, Arimura, Gen-ichiro, Urashimo, Soichi, Nishioka, Takaaki, and Takabayashi, Junji
- Subjects
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LIPOXYGENASES , *ARABIDOPSIS , *PARASITIC wasps , *PARASITISM , *BRASSICACEAE , *TRANSGENIC plants , *PIERIS rapae - Abstract
In response to herbivory by cabbage white butterfly (Pieris rapae) larvae, crucifer plants emit green leaf volatiles (GLVs) that attractCotesia glomerata, a carnivorous natural enemy of the larvae. To artificially increase GLV production by crucifers, we created transgenicArabidopsisthat constitutively expressed cucumber cytosolic lipoxygenase (CsLOX2). TransgenicArabidopsis(p35S::CsLOX2.6andp35S::CsLOX2.14) infested withP. rapaelarvae were more attractive toC. glomeratathan wild type (wt) and produced more jasmonic acid.p35S::CsLOX2.14had a higher incidence of parasitism of the larvae than did wt. Infested transgenic plants emitted more (Z)-3-hexenyl acetate, (E)-β-ocimene, (Z)-β-ocimene, and DMNT than infested wt plants; (Z)-3-hexenyl acetate is known to attractCotesiawasps. Transforming cruciferous crops with cytosolicCsLOX2could aid integrated pest management via tritrophic interactions. [ABSTRACT FROM PUBLISHER]
- Published
- 2013
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