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Metastatic pheochromocytoma and paraganglioma (MPPGL) is a rare neuroendocrine tumour in which genetic factors play an important role. In recent years, with the continuous progress of genetic testing technology, more and more susceptibility genes have been proved to be associated with MPPGL, making early identification of MPPGL possible. Recent studies have shown that genes associated with the development of MPPGL include SDHA, SDHB, SDHC, SDHD, SDHAF2, FH, MDH2, VHL, IDH1, PDH1/2, SLC25A11, GOT2, DLST, CSDE1, MAML3, H3F3A, MERTK, PCDHGC3, and KIF1B, with SDHA, SDHB, SDHC, SDHD, and SDHAF2 being the common pathogenic genes. Potential mutations affect the clinical manifestations of MPPGL, such as malignant potential and genetic prediction, which can help to better understand the clinical course and treat accordingly. Genetic testing for pheochromocytomas and paragangliomas allows for early detection of genetic syndromes and facilitates close follow-up of high-risk patients. This article provides a review of the progress of research on susceptibility genes identified in MPPGL in recent years, with a view to providing a certain theoretical basis for further related research. [ABSTRACT FROM AUTHOR]

Central nervous system(CNS)is a complex system closely related to anesthesiology. The molecular biological mechanisms of many CNS diseases were not exactly clear. Long noncoding RNA(lncRNA) H19 is abundant in human brain and plays an important role in the regulation of various CNS diseases. Besides, lncRNA H19 is also involved in the mechanism of several anesthetics. In this review, we emphasized the role and addressed the specific mechanisms of lncRNA H19 in CNS diseases and anesthetics, hoping to provide a new theoretical basis for future researches. [ABSTRACT FROM AUTHOR]

Objective To explore the relationship between SIRT1 genetic variation (rs1467568, rs4746720) and microscopic polyangiitis (MPA) in Guangxi population. Methods The SIRT1 polymorphisms of 208 patients with microscopic polyangiitis (MPA) and 211 healthy volunteers were genotyped by multiplex polymerase chain reaction (PCR) and high throughput sequencing. The allele frequencies and genotypes were compared. The linkage disequilibrium, haplotype and genetic model were analyzed. The relationship between different genotypes and clinical symptoms and laboratory biochemical indexes was evaluated. Results The results showed no significant difference between the two groups in genotype, allele frequency, and haplotype of SIRT1 rs1467568 and rs4746720(P > 0.05). In the age group ≥ 54 years old, the dominant model(OR = 0.50,95%CI: 0.27 ~ 0.92, P = 0.026), overdominant model(OA = 0.49,95%CI: 0.26 ~ 0.92, P = 0.026) and additive model (OR = 0.57, 95%CI: 0.33 ~ 0.99, P = 0.045) of SIRT1 rs1467568 were associated with lower susceptibility to MPA. In rs1467568, MPA patients with GG genotype had higher levels of glutamic pyruvic transaminase (P = 0.012), while in rs4746720, MPA patients with TT and TC genotypes had higher levels of glutamic oxaloacetic transaminase (P = 0.036). Conclusions In this study, SIRT1 rs4746720 was not related to the susceptibility to MPA in Guangxi population, but rs1467568 may be associated with the susceptibility to MPA in some people (5 54 years old) in Guangxi, and rs1467568 and rs4746720 were associated with the levels of glutamic pyruvic transaminase and glutamic oxaloacetic transaminase in patients with MPA. [ABSTRACT FROM AUTHOR]

Objective To investigate the protective effect of target temperature management based different durations with delayed hypothermia for traumatic brain injury(TBI) in rats. Methods 36 healthy adult male SD rats were randomly divided into NT group, HT4h group, HT24h group and HT48h group. The TBI model of rats was prepared with an electronic controllable cortical injury device. NT group was treated with normal temperature (37 ) 4 h after TBI, and the experimental groups were treated with low temperature (33.0 ± 1.0 ) 4 h after TBI for 4 h, 24 h, and 48 h. Three days after TBI, the motor function of the rats in each group was evaluated by beam walking test and inclined-grid climbing test, EB staining was used to measure the blood-brain barrier permeability, the change of hippocampal neurons was observed by Nissl staining, the expression of DCX and GFAP was detected by immunofluorescence, and the expression of Bcl-2 and Bax was measured by Western blot and immunohistochemistry. Results Compared with NT group, the experimental groups could significantly improve the motor function of TBI rats, reduce the permeability of blood-brain barrier,protect hippocampal neurons, promote DCX expression, inhibit GFAP expression, up-regulate the expression of Bcl-2 protein, and down-regulate the expression of Bax protein. However, the protective effect was more apparent in HT48h group than other experimental groups (P < 0.05). Conclusion Long-term delayed target temperature management has a significant brain protective effect. [ABSTRACT FROM AUTHOR]

Objective To investigate the role and mechanism of Rho-related GTP-binding protein F (RhoF)-mediated Th17 polarization in the development of severe acute pancreatitis (SAP). Methods RhoF transgenic mice were randomly divided into control group (n = 10), SAP group (n = 10) and SAP+Y-27632 group (n = 10), and WT mice were randomly divided into control group (n = 10), SAP group (n = 10) and SAP+Y-27632 group (n = 10). SAP models were established in all groups except the control group. The SAP+Y-27632 group was infused with Y-27632 via tail vein after model establishment. T cells in mouse blood samples were isolated for RhoF, p-MYPT1 protein detection and ROCK activity determination, and the percentage of lymphoid CD4+T cells producing IL-17 was analyzed by flow cytometry. Results The expression of RhoF and p-MYPT1 was increased in T cells in the SAP group compared with that in the control group (P < 0.01), and the level of RhoF was closely correlated with that of p-MYPT1 (P = 0.018). The expression of RhoF protein level in T cells of mice in the RhoF group increased by approximately 30% compared to that in the WT group, and the level of spontaneous IL-17 production by CD4+ T cells was significantly increased (P = 0.003). Compared with WT mice, the histological score of pancreatic injury, the expression of RhoF and p-MYPT1 in T cells, the number of IL-17+ T cells and serum IL-17 level in RhoF transgenic mice were significantly increased (P < 0.05). The histological score, RhoF and p-MYPT1 expression of T cells, IL-17+ T cell numbers and serum IL-17 level were significantly lower in the SAP+Y-27632 group than those in the SAP group (P < 0.05). Conclusion RhoF/ROCK signaling pathway-mediated polarization of Th17 cells is involved in the pathogenesis of SAP. [ABSTRACT FROM AUTHOR]

Objective To observe the expression of fructose-bisphosphate aldolase C (ALDOC) in colon cancer tissues and cells and to explore its effect on biological behavior. Methods GEPIA database was used to analyze the expression level of ALDOC in colon cancer tissues. The expression of ALDOC mRNA and protein in colon cancer tissues and cancer cells SW620, SW480, HT-29 and HCT-116 were detected by RT-qPCR and immu-nohistochemistry, and the relationship between ALDOC mRNA expression and clinicopathological factors was analyzed. Colon cancer cell SW620 was divided into control group, NC inhibitor group and ALDOC inhibitor group. ALDOC inhibitor or negative control was transfected into SW620 cells by lentivirus transfection kit. MTT assay and monoclonal formation of cancer cells were used to detect cell proliferation. Scratch test was used to detect cell migration ability, and transwell chamber test to detect the invasion ability of cells. The expression of ALDOC mRNA was detected by RT-qPCR, and that of ALDOC and MMP-2/9 was detected by western blot. Results GEPIA database showed that the expression level of ALDOC in colon cancer tissues was higher than that in normal tissues (P < 0.05). Compared with those in adjacent tissues or FHC, ALDOC mRNA and protein in colon cancer tissues and cells SW620, SW480, HT-29 and HCT-116 were up-regulated, and ALDOC mRNA level was significantly correlated with tumor differentiation, TNM stage and lymph node metastasis (P < 0.05). Down-regulating ALDOC mRNA could down-regulate MMP-2 and MMP-9 protein levels, inhibit cell growth, and reduce the number of wound healing and invasive cells (P < 0.05). Conclusion ALDOC level is up-regulated in colon cancer tissues and cells, and it is closely related to the poor prognosis of colorectal cancer patients. ALDOC regulates the invasion and migration of colon cancer cells by regulating MMP-2/9. [ABSTRACT FROM AUTHOR]