Search

Your search keyword '"Koomen, John"' showing total 12 results
Start Over Author "Koomen, John" Journal journal of proteome research
12 results on '"Koomen, John"'

2. Metabolic Changes Are Associated with Melphalan Resistance in Multiple Myeloma

Author

Koomen, David C., primary, Meads, Mark B., additional, Magaletti, Dario M., additional, Guingab-Cagmat, Joy D., additional, Oliveira, Paula S., additional, Fang, Bin, additional, Liu, Min, additional, Welsh, Eric A., additional, Meke, Laurel E., additional, Jiang, Zhijie, additional, Hampton, Oliver A., additional, Tungesvik, Alexandre, additional, De Avila, Gabriel, additional, Alugubelli, Raghunandan Reddy, additional, Nishihori, Taiga, additional, Silva, Ariosto S., additional, Eschrich, Steven A., additional, Garrett, Timothy J., additional, Koomen, John M., additional, and Shain, Kenneth H., additional

Published
2021
Full Text
View/download PDF

7. Mass Spectrometry Mapping of Epidermal Growth Factor Receptor Phosphorylation Related to Oncogenic Mutations and Tyrosine Kinase Inhibitor Sensitivity

Author

Zhang, Guolin, primary, Fang, Bin, additional, Liu, Richard Z., additional, Lin, Huiyi, additional, Kinose, Fumi, additional, Bai, Yun, additional, Oguz, Umut, additional, Remily-Wood, Elizabeth R., additional, Li, Jiannong, additional, Altiok, Soner, additional, Eschrich, Steven, additional, Koomen, John, additional, and Haura, Eric B., additional

Published
2011
Full Text
View/download PDF

8. Quantification of β-Catenin Signaling Components in Colon Cancer Cell Lines, Tissue Sections, and Microdissected Tumor Cells using Reaction Monitoring Mass Spectrometry

Author

Chen, Yi, primary, Gruidl, Mike, additional, Remily-Wood, Elizabeth, additional, Liu, Richard Z., additional, Eschrich, Steven, additional, Lloyd, Mark, additional, Nasir, Aejaz, additional, Bui, Marilyn M., additional, Huang, Emina, additional, Shibata, David, additional, Yeatman, Timothy, additional, and Koomen, John M., additional

Published
2010
Full Text
View/download PDF

11. Quantification of beta-catenin signaling components in colon cancer cell lines, tissue sections, and microdissected tumor cells using reaction monitoring mass spectrometry.

Author

Chen Y, Gruidl M, Remily-Wood E, Liu RZ, Eschrich S, Lloyd M, Nasir A, Bui MM, Huang E, Shibata D, Yeatman T, and Koomen JM

Subjects
Blotting, Western, Cell Line, Tumor, Chemical Fractionation, Chromatography, Liquid, Colonic Neoplasms metabolism, Electrophoresis, Polyacrylamide Gel, Humans, Colonic Neoplasms physiopathology, Mass Spectrometry methods, Signal Transduction physiology, beta Catenin metabolism
Abstract

Reaction monitoring mass spectrometry has emerged as a powerful tool for targeted detection and quantification of proteins in clinical samples. Here, we report the use of gel electrophoresis for protein fractionation and liquid chromatography coupled to multiple reaction monitoring mass spectrometry (LC-MRM) screening for quantitative analysis of components from the Wnt/beta-catenin signaling pathway, which contributes to colon tumor formation and progression. In silico tools are used to design LC-MRM screens for each target protein. Following successful peptide detection, stable isotope labeled peptides are synthesized and developed as internal standards. Then, the assays are implemented in colon cancer cell lines to achieve detection in minimal amounts of cells, compatible with direct translation to clinical specimens. Selected assays are compared with qualitative results from immunoblotting (Westerns) and translated to individual frozen colon tissue sections and laser capture microdissected tumor cells. This LC-MRM platform has been translated from in vitro models to clinical specimens, forming the basis for future experiments in patient assessment.

Published
2010
Full Text
View/download PDF

12. Proteome analysis of isolated perfused organ effluent as a novel model for protein biomarker discovery.

Author

Koomen JM, Wilson CR, Guthrie P, Androlewicz MJ, Kobayashi R, and Taegtmeyer H

Subjects
Animals, Biomarkers analysis, In Vitro Techniques, Male, Rats, Rats, Wistar, Myocardial Ischemia diagnosis, Myocardium chemistry, Perfusion, Proteins analysis, Proteome analysis, Proteomics
Abstract

The discovery of novel serological biomarkers is critical for improving disease diagnosis and monitoring treatment response. Proteomic analysis of model systems, such as isolated cells in culture and patient plasma and serum, represents the current state-of-the-art. Here, we coupled proteomics with isolated organ perfusion, which allows a disease state to be studied in a physiologic, yet controlled, environment. Potential markers specific to the disease or to changes in the surrounding tissue may be discovered. The effectiveness of this model was evaluated using proteomic analysis of effluent fractions collected from isolated beating rat hearts during reperfusion after brief episodes of ischemia. The detection of clinical markers for myocardial ischemia in this effluent was robust and analytically straightforward, validating the potential of isolated organ perfusion in diagnostic protein discovery.

Published
2006
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results