1. Combining proteomics and metabolite analyses to unravel cadmium stress-response in poplar leaves.
- Author
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Kieffer P, Planchon S, Oufir M, Ziebel J, Dommes J, Hoffmann L, Hausman JF, and Renaut J
- Subjects
- Amino Acid Sequence, Carbohydrates chemistry, Carbon chemistry, Chlorophyll chemistry, Models, Biological, Molecular Sequence Data, Photosynthesis drug effects, Plant Leaves drug effects, Polymers chemistry, Up-Regulation, Cadmium toxicity, Mitochondria metabolism, Oxygen Consumption, Populus drug effects, Populus growth & development, Proteomics methods
- Abstract
A proteomic analysis of poplar leaves exposed to cadmium, combined with biochemical analysis of pigments and carbohydrates revealed changes in primary carbon metabolism. Proteomic results suggested that photosynthesis was slightly affected. Together with a growth inhibition, photoassimilates were less needed for developmental processes and could be stored in the form of hexoses or complex sugars, acting also as osmoprotectants. Simultaneously, mitochondrial respiration was upregulated, providing energy needs of cadmium-exposed plants.
- Published
- 2009
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