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Start Over Author "Partier, A." Journal journal of proteomics
2 results on '"Partier, A."'

2. Identification of glycosyltransferases involved in cell wall synthesis of wheat endosperm

Author

Colette Larré, A. Partier, Dominique Tessier, Caroline Pont, Hélène Rogniaux, J. Marion, Brigitte Bouchet, Mathilde Francin-Allami, Fabienne Guillon, Anne-Laure Chateigner-Boutin, M. Suliman, Jérôme Salse, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Institut National de la Recherche Agronomique (INRA), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), inconnu, Inconnu, Univ Fed Santa Maria, Dept Solos, BR-97105900 Santa Maria, RS, Brazil, and Australia-India Council, CEPIA post-doc

Subjects
Dietary Fiber, Proteomics, 0106 biological sciences, [SDV]Life Sciences [q-bio], 01 natural sciences, Biochemistry, Mass Spectrometry, Endosperm, Cell Wall, Triticum, Plant Proteins, 2. Zero hunger, chemistry.chemical_classification, 0303 health sciences, MESH: Plant Proteins, Wheat endosperm, food and beverages, Wheat, MESH: Glycosyltransferases, symbols, Cell fractionation, Biophysics, MESH: Triticum, Biology, Polysaccharide, MESH: Endosperm, Cell wall, MESH: Golgi Apparatus, 03 medical and health sciences, symbols.namesake, MESH: Cell Wall, Polysaccharides, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Humans, 030304 developmental biology, MESH: Mass Spectrometry, MESH: Humans, Glycosyltransferases, Golgi apparatus, Fusion protein, MESH: Polysaccharides, chemistry, MESH: Dietary Fiber, Subcellular fraction, Biogenesis, 010606 plant biology & botany
Abstract

International audience; Plant cell walls are complex structures critical for plant fitness and valuable for human nutrition as dietary fiber and for industrial uses such as biofuel production. The cell wall polysaccharides in wheat endosperm consist of two major polymers, arabinoxylans and beta-glucans, as well as other minor components. Most of these polysaccharides are synthesized in the Golgi apparatus but the mechanisms underlying their synthesis have yet to be fully elucidated and only a few of the enzymes involved have been characterized. To identify actors involved in the wheat endosperm cell wall formation, we used a subcellular fractionation strategy to isolate Golgi-enriched fractions from endosperm harvested during active cell wall deposition. The proteins extracted from these Golgi-enriched fractions were analyzed by LC-MS/MS. We report the identification of 1135 proteins among which 64 glycosyltransferases distributed in 17 families. Their potential function in cell wall synthesis is discussed. In addition, we identified 63 glycosylhydrolases, some of which may be involved in cell wall remodeling. Several glycosyltransferases were validated by showing that when expressed as fusion proteins with a fluorescent reporter, they indeed accumulate in the Golgi apparatus. Our results provide new candidates potentially involved in cell wall biogenesis in wheat endosperm.

Published
2013
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