1. Identification of SNPs in Beta 2 Microglobulin (β2M) Gene and their Association with IgG Concentration in Neonatal Buffalo Calves.
- Author
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Chaudhary, Rajni, Kumar, Subodh, Yathish, H. M., A., Sivakumar, Mishra, Chinmoy, Kumar, Amit, Chauhan, Anuj, Sivamani, B., and Sahoo, N. R.
- Subjects
MICROGLOBULINS ,CALVES ,POLYMERASE chain reaction ,HAPLOTYPES ,ENZYME-linked immunosorbent assay ,ANIMAL health - Abstract
The present study was taken up to find out SNPs in Beta 2 Microglobulin (β2M) gene and their possible association with serum IgG concentrations of newborn buffalo calves. Forty newborn Murrah buffaloes calves were included in the present study. Polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) technique was used to explore the polymorphism in β2M gene of the buffalo calves. The PCR-SSCP analysis and sequencing of different patterns obtained by silver staining of various exonic and intronic fragments revealed nine SNPs. All the identified SNPs were used to prepare the haplotypes for the calves. Fourteen different haplotypes were observed. Indirect ELISA was done to estimate IgG concentrations in serum samples of the calves. IgG levels in calf serum ranged from 0.25 to 14.88 mg/ml and mean IgG concentration in serum was estimated as 11.68 ± 0.75 mg/ml. The least square analysis of variance could not exhibit a significant effect of calf haplotype on serum IgG concentration. [ABSTRACT FROM AUTHOR]
- Published
- 2016