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Introduction: Acinetobacter baumannii known as a main agent in nosocomial infections and its drug resistance is a medical problem in hospitals. In the present study, growth control of Acinetobacter by Oregano essential oil was investigated in-vitro and in experimental wounds on rats. Materials and Methods: Oregano essential oil (OES) was prepared by a Clevenger apparatus in a hydrodistillation process. Susceptibility of clinical isolate of Acinetobacterto essential oil was evaluated by disk diffusion and microbroth dilution methods. Ketamine and xylazine were used for surgical anesthesia induction in rats. The backs of 30 rats were shaved and an experimental wound in one square inch was prepared between two scapulae. All the ulcers were infected with fresh culture of Acinetobacter. The rats were divided into three equal groups for treatment by OES twice a day, treatment with silversulfuridine ointment (SSO) and control group without any treatment. Infection symptoms were assessed until the wound was fully granulated. Results: MIC and MBC were as 4 mg/ml and 6 mg/ml, respectively. Blood culture of control group and SSO contained A. baumannii but was negative in treatment group. The cultivation and examination of the wounds and its secretions revealed the absence of A. baumannii in treatment group. Wound healing was faster in the treatment group than in the control groups. Conclusion: Infection of the wounds infected with A. baumannii was interestingly controlled by OES and subsequently accelerated the wound healing process in Wistar rats. Therefore, OES recommends for evaluation in a clinical trial.