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Osteochondral lesion of the talus (OLT) is a common cause of ankle pain that often occurs in the talar dome and leads to talar cartilage and subchondral bone damage. Osteochondral autograft transplantation is a logical treatment option. It is known that if the cartilage does not heal properly after injury, it degenerates, and osteoarthritis worsens. A three dimensional (3D)-printed guide plate can be used to find the curved articular surface from the donor site which optimally fits the defect in the talus. Herein, we present the case of a 28-year-old man who had an open injury from the crash of a tricycle in the right ankle at the age of 5. Radiographs revealed a large defect in the medial talar dome that affected nearly half of the talar dome. We performed the debridement of the ankle lesion. An osteochondral autograft was harvested from the medial femoral condyle (MFC) with the help of a personalised 3D-printed guide plate. This 3D-printed guide plate simulated the contour of a specific area in the talar dome, which involved the site of the defect. The autograft was then transplanted into the talus defect. The efficacy of this technique was evaluated at 2, 4, and 7 months after surgery and proven to be reliable.