1. Simultaneous determination of 5 alpha reduced metabolites of testosterone in human plasma
- Author
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F. Sciarra, M.V. Adamo, Vincenzo Toscano, S. Foli, Elisa Petrangeli, and S. Caiola
- Subjects
Adult ,Male ,medicine.medical_specialty ,Radioimmunoassay ,Alpha (ethology) ,Biochemistry ,Endocrinology ,Internal medicine ,medicine ,Animals ,Humans ,Testosterone ,Young male ,Aged ,Chromatography ,Chemistry ,Dihydrotestosterone ,Middle Aged ,Androgen Metabolism ,Peripheral ,Human plasma ,Rabbits ,Oxidation-Reduction ,medicine.drug - Abstract
17β-Hydroxy-4-androsten-3-one (testosterone), 17β-hydroxy-5α-androstan-3-one (dihydrotestosterone), 3α,17β-dihydroxy-5α-androstane (3αadiol) and 3β,17β-dihydroxy-5α-androstane (3βadiol) were measured simultaneously in the same sample of human peripheral plasma using celite microcolumn chromatography and two antisera (anti testosterone-6-carboxy-methyl-oxime BSA for testosterone and anti 5α-dihydrotestosterone-l-carboxy-ethyl-thioether BSA for dihydrotestosterone, Sαadiol and 3βadiol). The concentrations (ng/dl ± SD) of the four androgens in normal young males and in normal females are respectively: 610.33 ± 137.94 and 30.4 ± 9.1 for testosterone, 47.8 ± 9.4 and 16.8 ± 6.7 for dihydrotestosterone, 28.7 ± 8.6 and 10.8 ± 3.03 for 3αadiol and 54.9 ± 29 and 23.9 ± 6.6 for 3βadiol. These values decrease significantly in elderly males. Results obtained in orchiectomized patients and in hirsute women suggest that the determination of 5α reduced metabolites in peripheral plasma may be a useful clinical parameter for the evaluation of peripheral androgen metabolism.
- Published
- 1981