1. Developing VUV spectroscopy for protein folding and material luminescence on beamline 4B8 at the Beijing Synchrotron Radiation Facility
- Author
-
Hao Zhuang, Daowu Li, Yan Huang, Shuaishuai Sun, Yinglei Tan, Ai-Yu Zhou, Ye Tao, and Zhenghua Gao
- Subjects
Protein Folding ,Nuclear and High Energy Physics ,Circular dichroism ,Radiation ,Myoglobin ,business.industry ,Chemistry ,Circular Dichroism ,Analytical chemistry ,Synchrotron radiation ,Fluorescence ,Fluorescence spectroscopy ,Spectrometry, Fluorescence ,Beamline ,Luminescent Measurements ,Humans ,Optoelectronics ,Spectrophotometry, Ultraviolet ,Time-resolved spectroscopy ,Luminescence ,Spectroscopy ,business ,Instrumentation ,Serum Albumin ,Synchrotrons - Abstract
The new 4B8 beamline provides UV-VUV light in the wavelength range from 360 to 120 nm. It uniquely enables two kinds of spectroscopy measurements: synchrotron radiation circular dichroism spectroscopy and VUV excited fluorescence spectroscopy. The former is mainly used in protein secondary structure studies, and the latter in VUV excited luminescent materials research. Remote access to fluorescence measurement has been realised and users can collect data online. Besides steady-state measurements, fluorescence lifetime measurements have been established using the time domain method, while a laser-induced temperature jump is under development for protein folding dynamics using circular dichroism as a probe.
- Published
- 2009
- Full Text
- View/download PDF