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Combination breeding and mutation breeding are widely used methods in plant breeding. Intensive studies are carried out on biotechnological methods that will allow obtaining homozygous lines in a short time in populations obtained with these two techniques. Numerous studies have been carried out by different researchers on mutation breeding in wheat. There are few studies on anther culture response in mutagen-treated genotypes. Two different bread wheat promising advanced lines were used as the material in the study, in which the possibilities of combining mutation breeding with anther culture through ionizing radiation in high quality wheat breeding were investigated. In the study, the responses of advanced bread wheat mutant lines to anther culture, to which eight different doses of gamma rays (0, 100, 150, 200, 250, 300, 350, 400 Gy) were applied, including the control, were investigated. There are significant differences between genotypes and irradiation doses for all traits studied. It has been shown that it is possible to decrease albinism and increase the response of anther culture with dose-dependent gamma irradiation depending on bread wheat varieties. In the multiple comparison test to classify the difference between doses; statistically, 150 gray dose 5.60 is in the first statistical class and in the first place with the number of transferred green plants. After that, it ranks second in the same class with the number of green plants transferred with a 300 gray dose of 5.21. In the total number of regenerated green plants excluding controls (888), 635 unit (71.5%) and 205 unit (23.1%) haploids and spontaneous double haploid plants were obtained, respectively. In the study integrated into the bread wheat breeding program, a total of 205 spontaneous double haploid mutant lines were produced. According to the data obtained, it was shown that the gamma ray doses of 150 and 200 Gy had a significant stimulation effect on all parameters studied and ultimately the success index of anther culture in bread wheat compared to control. [ABSTRACT FROM AUTHOR]

The study was carried out with a total of 24 bread wheat genotypes including 20 advanced lines and 4 check varieties (Gerek79, Carisma, Bayraktar2000, and Artico) with soft grain structure and four replications according to the randomized complete block design in Hamidiye and Karabayır locations of Eskişehir during the 2014-2015 growing season. In the study, grain yield, thousand grain weight, test weight, kernel hardness, Zeleny sedimentation value, ash content and protein content were investigated in the genotypes. According to the results of the analysis of variance performed for the traits examined in the study, the differences between location, bread wheat genotypes and interaction averages were found to be statistically significant, except for Zeleny sedimentation value and protein content, respectively. It is understood that there is a wide variation between genotypes and locations for other traits, except for the protein ratio trait. While location effects are higher than genotype effects for thousand grain weight, test weight, ash content and protein content, genotype effects are higher than location for single kernel characterization system and Zeleny sedimentation value. The mean grain yield of biscuit wheat genotypes ranged from 4425 kg ha-1 to 2000 kg ha-1 . Considering the trial quality results; it was observed that there was a high rate of variation between genotypes for thousand grain weight (32.67-48.61 g), test weight (82.40-75.25 kg hl-1 ), kernel hardness (17.08-39.68), Zeleny sedimentation value (20.63-33.00 ml) and ash content (0.478% - 0.610%), respectively. While BİS-3, BİS-6, BİS-9, BİS-18, BİS-21 and BİS-22 were bestperformed lines for grain yield, BİS-3, BİS-9, BİS-11, BİS-12, BİS-13 and BİS-19 were the prominent lines for biscuit-making quality trait. It was concluded that it would be appropriate to include these lines in yield trials before registration as a variety of candidates. Keywords: Soft bread wheat, Grain yield, Kernel ch [ABSTRACT FROM AUTHOR]

The present research was conducted to evaluate the M1, M2, M3 and M4 bread wheat (Triticum aestivum L.) mutant populations for yield and yield related traits during 2010-11, 2011-12, 2012-13 and 2013-14 at Tekirdağ ecological conditions. Three wheat genotypes were treated with different levels of gamma rays (100 Gy, 200 Gy, 300 Gy, 400 Gy, 500 Gy and Control). The mutated plants were evaluated along with parental lines (control) for grain yield (GY) and its contributing traits such as plant height (PH), spike length (SL), the number of spikelets per spike (NSPS), the number of grains per spike (NGPS), grain weight per spike (GWPS), harvest index (HI) and thousand grain weight (TGW) under field conditions. The results obtained from the present study showed that the genotypes significantly and variably differed in their response for various traits at different gamma rays doses. The traits such as PH, TGW and grain yield (GY) showed generally reduction with higher gamma irradiation doses as compared to low doses, while mutagenic treatments shifted the mean values mostly towards the negative direction in the other yield components. But, the negative or positive shifts were not unidirectional or equally effective for all the traits. These findings suggested that the variability could be induced through the use of gamma irradiations in bread wheat. Some of the traits showed improvement due to the induced mutations could be used in future wheat breeding programs. The differences in mean values and the nature of variability observed in M2 indicated a possible preference of selection in M3 generation. [ABSTRACT FROM AUTHOR]

The study was carried out in randomized block design with 20 advanced lines with soft grain structure by developed Eskişehir Agricultural Research Institute and 4 standard wheat genotypes in Hamidiye and Karabayır locations of Eskişehir Province in the 2014-2015 growing period. The solvent retention capacity (SRC) tests (sodium carbonate (5%), sucrose (50%), lactic acid (5%) and distilled water) and gluten performance index (GPI) were investigated for 20 lines and 4 standard varieties in the study. According to the results of the analysis of variance regarding SRC tests and GPI, the differences between the genotypes, locations and their interaction averages were found to be statistically significant. The fact that the size of the variation between locations is higher than the genotype and interaction variances indicates that there are significant differences between the locations selected for the study, and the low interaction variance shows that the locations are correctly selected for the studied genotypes and their number is sufficient. For SRC tests used in soft wheat milling and biscuit quality determination, it was determined that the genotype and location effects were greater than the genotype x location interaction effects. The mean values of genotypes ranged between 86.68% and 112.83% for lactic acid SRC, 79.95% and 89.29% for sucrose SRC, 79.52% and 94.83% for sodium carbonate SRC and 58.18% to 65.14% for water SRC. The genotypes taken into the study in terms of GPI had averages varying between 0.49 and 0.66. Considering the obtained SRC tests and GPI reference values, it was determined that BİS-19 could be the line with the best biscuit performance. The BİS-2, BİS-11, BİS-13 and BİS-16 were other promising lines for biscuit quality performances. In addition, BİS-9, BİS-17, BİS-22 and BİS-8 have been promising lines that can be evaluated in breeding studies for biscuit quality. [ABSTRACT FROM AUTHOR]