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Start Over Author "Horch, Marius" Journal journal of the american chemical society
5 results on '"Horch, Marius"'

1. Reversible Glutamate Coordination to High-Valent Nickel Protects the Active Site of a [NiFe] Hydrogenase from Oxygen

Author

Kulka-Peschke, Catharina J., primary, Schulz, Anne-Christine, additional, Lorent, Christian, additional, Rippers, Yvonne, additional, Wahlefeld, Stefan, additional, Preissler, Janina, additional, Schulz, Claudia, additional, Wiemann, Charlotte, additional, Bernitzky, Cornelius C. M., additional, Karafoulidi-Retsou, Chara, additional, Wrathall, Solomon L. D., additional, Procacci, Barbara, additional, Matsuura, Hiroaki, additional, Greetham, Gregory M., additional, Teutloff, Christian, additional, Lauterbach, Lars, additional, Higuchi, Yoshiki, additional, Ishii, Masaharu, additional, Hunt, Neil T., additional, Lenz, Oliver, additional, Zebger, Ingo, additional, and Horch, Marius, additional

Published
2022
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2. Shedding Light on Proton and Electron Dynamics in [FeFe] Hydrogenases

Author

Lorent, Christian, primary, Katz, Sagie, additional, Duan, Jifu, additional, Kulka, Catharina Julia, additional, Caserta, Giorgio, additional, Teutloff, Christian, additional, Yadav, Shanika, additional, Apfel, Ulf-Peter, additional, Winkler, Martin, additional, Happe, Thomas, additional, Horch, Marius, additional, and Zebger, Ingo, additional

Published
2020
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5. Reversible Active Site Sulfoxygenation Can Explain the Oxygen Tolerance of a NAD+-Reducing [NiFe] Hydrogenase and Its Unusual Infrared Spectroscopic Properties.

Author

Horch, Marius, Lauterbach, Lars, Mroginski, Maria Andrea, Hildebrandt, Peter, Lenz, Oliver, and Zebger, Ingo

Subjects
*PHOTOSYNTHETIC oxygen evolution, *OXYGEN compounds, *NONMETALS, *HYDROGENASE, *REFRIGERANTS
Abstract

Oxygen-tolerant [NiFe] hydrogenases are metalloenzymes that represent valuable model systems for sustainable H2 oxidation and production. The soluble NAD+-reducing [NiFe] hydrogenase (SH) from Ralstonia eutropha couples the reversible cleavage of H2 with the reduction of NAD+ and displays a unique O2 tolerance. Here we performed IR spectroscopic investigations on purified SH in various redox states in combination with density functional theory to provide structural insights into the catalytic [NiFe] center. These studies revealed a standard-like coordination of the active site with diatomic CO and cyanide ligands. The long-lasting discrepancy between spectroscopic data obtained in vitro and in vivo could be solved on the basis of reversible cysteine oxygenation in the fully oxidized state of the [NiFe] site. The data are consistent with a model in which the SH detoxifies O2 catalytically by means of an NADH-dependent (per)oxidase reaction involving the intermediary formation of stable cysteine sulfenates. The occurrence of two catalytic activities, hydrogen conversion and oxygen reduction, at the same cofactor may inspire the design of novel biomimetic catalysts performing H2-conversion even in the presence of O2. [ABSTRACT FROM AUTHOR]

Published
2015
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