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In this study, detailed morphological and anatomical features of Isatis quadrialata were examined. With anatomical analysis, transverse sections of root, stem and leaf were examined. Epidermis is a protective tissue in root transverse sections. Transverse sections of stem contain one layered epidermis, cortex parenchyma, vascular bundles and pith cells. The cross sections of leaves have two layered palisade parenchyma and spongy parenchyma with bifacial mesophyll. Transverse sections of fruit have one layered exocarp, multi layered mesocarp and endocarp. In addition, fruit and seed micromorphologies were studied. The fruit surface ornamentation is reticulate while the seed surface ornamentation is blister. The anatomical characteristics of Isatis quadrialata were determined of taxonomic importance. [ABSTRACT FROM AUTHOR]