1. Human platelet alloantigens HPA-1, HPA-2, and HPA-3 polymorphisms associated with extent of severe coronary artery disease
- Author
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Nesrine Abboud, Fatma Anabi, Touhami Mahjoub, Brahim Nsiri, Ali Ben Khalfallah, Wassim Y. Almawi, Faouzi Added, Sonia Ben-Hadj-Khalifa, and Lakdhar Ghazouani
- Subjects
Male ,endocrine system ,Coronary Artery Disease ,Disease ,Coronary artery disease ,Risk Factors ,Genotype ,Humans ,Medicine ,Antigens, Human Platelet ,Genetic Predisposition to Disease ,Allele ,Genotyping ,Polymorphism, Single-Stranded Conformational ,Aged ,Retrospective Studies ,biology ,business.industry ,Haplotype ,Hematology ,Middle Aged ,medicine.disease ,Human platelet antigen ,Haplotypes ,Immunology ,biology.protein ,Female ,Gene polymorphism ,Cardiology and Cardiovascular Medicine ,business ,hormones, hormone substitutes, and hormone antagonists - Abstract
The contribution of human platelet antigen (HPA)-1 (GPIIb/IIIa), HPA-2 (GPIb/IX), and HPA-3 (GPIIb/IIIa) polymorphisms to the risk of coronary artery disease (CAD) was investigated in 341 CAD patients and 316 matched control subjects. HPA genotyping was performed by PCR-SSP. Regression analysis was employed in assessing the contribution of these variants to CAD risk. The frequency of HPA-1b (P = .009) and HPA-3b (P = .004) alleles, and HPA-1a/1b (P = .045), HPA-1b/1b (P = .007), and HPA-3b/3b (P = .008) genotypes were higher in patients than control subjects. No significant association was demonstrated between the HPA variants and 1-, 2- and 3-vessel disease. HPA-1b/2a/3b (Pc = .021) and HPA-1b/2b/3a (Pc = .002) haplotypes were positively associated with CAD, thereby conferring a disease susceptibility nature to these haplotypes. Multivariate analysis confirmed the positive association of HPA-1b/2a/3b (aOR = 3.72; 95% CI = 1.49-9.28), and in addition identified HPA-1b/2a/3a (aOR = 2.49; 95% CI = 1.06-5.86) to be positively associated with CAD, after adjusting for a number of covariates. Our results demonstrate positive association of HPA variants and specific HPA-1/HPA-2/HPA-3 haplotypes with CAD in Tunisians.
- Published
- 2009