1. Investigation of the caspase-dependent mitochondrial apoptotic pathway in mononuclear cells of patients with systemic lupus erythematosus
- Author
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Wei-Che Lin, Chih-Min Su, Ben-Chung Cheng, Yi-Fang Chiang, Ya-Ting Chang, Chia-Te Kung, Cheng-Hsien Lu, Hung-Chen Wang, Wen-Neng Chang, Chung-Jen Chen, Tien-Tsai Cheng, Yu-Jih Su, Chih-Cheng Huang, Yu-Jun Lin, and Nai-Wen Tsai
- Subjects
Adult ,Male ,medicine.medical_specialty ,Lymphocyte ,Apoptosis ,Biology ,Peripheral blood mononuclear cell ,General Biochemistry, Genetics and Molecular Biology ,Monocytes ,Systemic lupus erythematosus ,Annexin ,Interferon ,immune system diseases ,Internal medicine ,medicine ,Humans ,Lupus Erythematosus, Systemic ,Lymphocytes ,skin and connective tissue diseases ,Caspase 10 ,Adaptor Proteins, Signal Transducing ,Demography ,Medicine(all) ,Lupus erythematosus ,Biochemistry, Genetics and Molecular Biology(all) ,Research ,Leukocyte apoptosis ,General Medicine ,Middle Aged ,medicine.disease ,Caspase ,Caspase 9 ,Mitochondria ,Endocrinology ,medicine.anatomical_structure ,Case-Control Studies ,Immunology ,Leukocytes, Mononuclear ,Female ,Biomarkers ,medicine.drug ,Interferon regulatory factors - Abstract
Background This study aimed to explore the role of apoptosis initiators, caspase-9, caspase-10, mitochondrial anti-viral signaling protein (MAVS), and interferon regulatory factor 7 (pIRF7), in patients with systemic lupus erythematosus (SLE). Methods Leukocyte apoptosis was determined by flow cytometry, including annexin V, APO2.7, and 7-amino-actinomycin D (7-AAD) on each subtype of leukocyte in 35 patients with SLE, 15 disease controls, and 17 volunteer normal controls. Levels of caspase-9, caspase-10, MAVS, and pIRF7 in mononuclear cells and the disease activity index (SLEDAI) in the SLE patients were determined. Correlation among intracellular adaptor proteins and caspase levels were calculated. Results The SLE patients had higher APO2.7 in total leukocyte, lymphocyte, and monocytes, and higher late apoptosis markers in total leukocytes and neutrophils than normal controls (all p
- Published
- 2014