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Your search keyword '"Enteropathy-Associated T-Cell Lymphoma"' showing total 4 results
Start Over Descriptor "Enteropathy-Associated T-Cell Lymphoma" Journal journal of veterinary internal medicine
4 results on '"Enteropathy-Associated T-Cell Lymphoma"'

1. Characterization of the intestinal mucosal proteome in cats with inflammatory bowel disease and alimentary small cell lymphoma

Author

Marsilio, Sina, Dröes, Floris C, Dangott, Lawrence, Chow, Betty, Hill, Steve, Ackermann, Mark, Estep, J Scott, Lidbury, Jonathan A, Suchodolski, Jan S, and Steiner, Jörg M

Subjects
Veterinary Sciences, Agricultural, Veterinary and Food Sciences, Digestive Diseases, Rare Diseases, Prevention, Detection, screening and diagnosis, 4.1 Discovery and preclinical testing of markers and technologies, Animals, Inflammatory Bowel Diseases, Intestinal Mucosa, Leukemia, Lymphocytic, Chronic, B-Cell, Lymphoma, Non-Hodgkin, Proteome, EATL, enteropathy-associated T-cell lymphoma, feline chronic enteropathy, Veterinary sciences
Abstract

BackgroundCurrent tests for diagnosis and differentiation of lymphoplasmacytic enteritis (LPE) and small cell lymphoma (SCL) in cats are expensive, invasive, and lack specificity. The identification of less invasive, more reliable biomarkers would facilitate diagnosis.ObjectivesTo characterize the mucosal proteome in endoscopically obtained, small intestinal tissue biopsy specimens. We hypothesized that differentially expressed proteins could be identified and serve as biomarker candidates for the differentiation of LPE and SCL in cats.AnimalsSix healthy control cats, 6 cats with LPE, and 8 cats with SCL.MethodsThe mucosal proteome was analyzed using 2-dimensional fluorescence difference gel electrophoresis (2D DIGE) and nanoflow liquid chromatography tandem mass spectrometry. For 5 proteins, results were verified by Western blot analysis.ResultsA total of 2349 spots were identified, of which 9 were differentially expressed with a ≥2-fold change between healthy cats and cats with LPE and SCL (.01 < P

Published
2021

2. Differentiation of lymphocytic‐plasmacytic enteropathy and small cell lymphoma in cats using histology‐guided mass spectrometry

Author

Marsilio, Sina, Newman, Shelley J, Estep, James Scot, Giaretta, Paula R, Lidbury, Jonathan A, Warry, Emma, Flory, Andi, Morley, Paul S, Smoot, Katy, Seeley, Erin H, Powell, Matthew J, Suchodolski, Jan S, and Steiner, Jörg M

Subjects
Veterinary Sciences, Agricultural, Veterinary and Food Sciences, Rare Diseases, Cancer, Animals, Cat Diseases, Cats, Enteropathy-Associated T-Cell Lymphoma, Immunohistochemistry, Leukemia, Lymphocytic, Chronic, B-Cell, Mass Spectrometry, Retrospective Studies, Sensitivity and Specificity, HGMS, MALDI mass spectrometry, PARR, PCR for antigen receptor rearrangements, chronic enteropathy, clonality testing, feline, inflammatory bowel disease, inflammatory enteropathy, small cell lymphoma, Veterinary sciences
Abstract

BackgroundDifferentiation of lymphocytic-plasmacytic enteropathy (LPE) from small cell lymphoma (SCL) in cats can be challenging.Hypothesis/objectiveHistology-guided mass spectrometry (HGMS) is a suitable method for the differentiation of LPE from SCL in cats.AnimalsForty-one cats with LPE and 52 cats with SCL.MethodsThis is a retrospective clinicopathologic study. Duodenal tissue samples of 17 cats with LPE and 22 cats with SCL were subjected to HGMS, and the acquired data were used to develop a linear discriminate analysis (LDA) machine learning algorithm. The algorithm was subsequently validated using a separate set of 24 cats with LPE and 30 cats with SCL. Cases were classified as LPE or SCL based on a consensus by an expert panel consisting of 5-7 board-certified veterinary specialists. Histopathology, immunohistochemistry, and clonality testing were available for all cats. The panel consensus classification served as a reference for the calculation of test performance parameters.ResultsRelative sensitivity, specificity, and accuracy of HGMS were 86.7% (95% confidence interval [CI]: 74.5%-98.8%), 91.7% (95% CI: 80.6%-100%), and 88.9% (95% CI: 80.5%-97.3%), respectively. Comparatively, the clonality testing had a sensitivity, specificity, and accuracy of 85.7% (95% CI: 72.8%-98.7%), 33.3% (95% CI: 14.5%-52.2%), and 61.5% (95% CI: 48.3%-74.8%) relative to the panel decision.Conclusions and clinical importanceHistology-guided mass spectrometry was a reliable technique for the differentiation of LPE from SCL in duodenal formalin-fixed paraffin-embedded samples of cats and might have advantages over tests currently considered state of the art.

Published
2020

3. Characterization of the intestinal mucosal proteome in cats with inflammatory bowel disease and alimentary small cell lymphoma

Author

Sina Marsilio, Floris C. Dröes, Lawrence Dangott, Betty Chow, Steve Hill, Mark Ackermann, J. Scott Estep, Jonathan A. Lidbury, Jan S. Suchodolski, and Jörg M. Steiner

Subjects
EATL, enteropathy‐associated T‐cell lymphoma, feline chronic enteropathy, Veterinary medicine, SF600-1100
Abstract

Abstract Background Current tests for diagnosis and differentiation of lymphoplasmacytic enteritis (LPE) and small cell lymphoma (SCL) in cats are expensive, invasive, and lack specificity. The identification of less invasive, more reliable biomarkers would facilitate diagnosis. Objectives To characterize the mucosal proteome in endoscopically obtained, small intestinal tissue biopsy specimens. We hypothesized that differentially expressed proteins could be identified and serve as biomarker candidates for the differentiation of LPE and SCL in cats. Animals Six healthy control cats, 6 cats with LPE, and 8 cats with SCL. Methods The mucosal proteome was analyzed using 2‐dimensional fluorescence difference gel electrophoresis (2D DIGE) and nanoflow liquid chromatography tandem mass spectrometry. For 5 proteins, results were verified by Western blot analysis. Results A total of 2349 spots were identified, of which 9 were differentially expressed with a ≥2‐fold change between healthy cats and cats with LPE and SCL (.01 < P

Published
2021
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4. Differentiation of lymphocytic‐plasmacytic enteropathy and small cell lymphoma in cats using histology‐guided mass spectrometry

Author

Jan S. Suchodolski, Sina Marsilio, Andi Flory, Erin H. Seeley, Shelley J. Newman, Jonathan A. Lidbury, Katy Smoot, James Scot Estep, Paul S. Morley, Paula R. Giaretta, Matthew J. Powell, Jörg M. Steiner, and Emma Warry

Subjects
medicine.medical_specialty, 040301 veterinary sciences, Standard Article, 030204 cardiovascular system & hematology, Cat Diseases, Small cell lymphoma, Gastroenterology, PARR, Sensitivity and Specificity, Mass Spectrometry, 0403 veterinary science, 03 medical and health sciences, PCR for antigen receptor rearrangements, 0302 clinical medicine, Enteropathy-Associated T-Cell Lymphoma, inflammatory bowel disease, Internal medicine, medicine, Animals, clonality testing, Enteropathy, feline, HGMS, Retrospective Studies, CATS, lcsh:Veterinary medicine, General Veterinary, business.industry, Histology, 04 agricultural and veterinary sciences, Chronic enteropathy, medicine.disease, small cell lymphoma, Immunohistochemistry, Leukemia, Lymphocytic, Chronic, B-Cell, Confidence interval, Standard Articles, chronic enteropathy, MALDI mass spectrometry, Cats, lcsh:SF600-1100, Histopathology, Test performance, SMALL ANIMAL, business, inflammatory enteropathy
Abstract

Background Differentiation of lymphocytic-plasmacytic enteropathy (LPE) from small cell lymphoma (SCL) in cats can be challenging. Hypothesis/objective Histology-guided mass spectrometry (HGMS) is a suitable method for the differentiation of LPE from SCL in cats. Animals Forty-one cats with LPE and 52 cats with SCL. Methods This is a retrospective clinicopathologic study. Duodenal tissue samples of 17 cats with LPE and 22 cats with SCL were subjected to HGMS, and the acquired data were used to develop a linear discriminate analysis (LDA) machine learning algorithm. The algorithm was subsequently validated using a separate set of 24 cats with LPE and 30 cats with SCL. Cases were classified as LPE or SCL based on a consensus by an expert panel consisting of 5-7 board-certified veterinary specialists. Histopathology, immunohistochemistry, and clonality testing were available for all cats. The panel consensus classification served as a reference for the calculation of test performance parameters. Results Relative sensitivity, specificity, and accuracy of HGMS were 86.7% (95% confidence interval [CI]: 74.5%-98.8%), 91.7% (95% CI: 80.6%-100%), and 88.9% (95% CI: 80.5%-97.3%), respectively. Comparatively, the clonality testing had a sensitivity, specificity, and accuracy of 85.7% (95% CI: 72.8%-98.7%), 33.3% (95% CI: 14.5%-52.2%), and 61.5% (95% CI: 48.3%-74.8%) relative to the panel decision. Conclusions and clinical importance Histology-guided mass spectrometry was a reliable technique for the differentiation of LPE from SCL in duodenal formalin-fixed paraffin-embedded samples of cats and might have advantages over tests currently considered state of the art.

Published
2020

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