1. Recombinant phosphoprotein based single serum dilution ELISA for rapid serological detection of Newcastle disease virus.
- Author
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Das, Moushumee and Kumar, Sachin
- Subjects
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PHOSPHOPROTEINS , *NEWCASTLE disease virus , *ENZYME-linked immunosorbent assay , *COMMUNICABLE diseases , *PARAMYXOVIRUSES , *SEROTYPES - Abstract
Newcastle disease virus (NDV) is the causative agent of a highly contagious disease in avian species. All strains of NDV belong to avian paramyxovirus serotype-1. The disease is endemic in different parts of the world and vaccination is the only way to protect birds from NDV infection. The virus non-structural phosphoprotein (P) is the second most abundant protein and a major modulator of viral replication. Although P protein shows lesser evolutionary divergence among NDV isolates, it is known to be highly divergent among different avian paramyxovirus serotypes. In the present study, a recombinant P protein based single serum dilution ELISA was developed which showed better sensitivity, specificity and accuracy as compared to conventional methods for NDV detection. The recombinant P protein based ELISA could be an alternative to existing diagnostics against NDV infection in chickens. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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