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Your search keyword '"Hoffmann, Markus"' showing total 12 results
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12 results on '"Hoffmann, Markus"'

1. Rapid SARS-CoV-2 Adaptation to Available Cellular Proteases

Author

Chaudhry, M. Zeeshan, primary, Eschke, Kathrin, additional, Hoffmann, Markus, additional, Grashoff, Martina, additional, Abassi, Leila, additional, Kim, Yeonsu, additional, Brunotte, Linda, additional, Ludwig, Stephan, additional, Kröger, Andrea, additional, Klawonn, Frank, additional, Pöhlmann, Stefan H., additional, and Cicin-Sain, Luka, additional

Published
2022
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3. Tetherin Inhibits Nipah Virus but Not Ebola Virus Replication in Fruit Bat Cells

Author

Hoffmann, Markus, primary, Nehlmeier, Inga, additional, Brinkmann, Constantin, additional, Krähling, Verena, additional, Behner, Laura, additional, Moldenhauer, Anna-Sophie, additional, Krüger, Nadine, additional, Nehls, Julia, additional, Schindler, Michael, additional, Hoenen, Thomas, additional, Maisner, Andrea, additional, Becker, Stephan, additional, and Pöhlmann, Stefan, additional

Published
2019
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8. The Tetherin Antagonism of the Ebola Virus Glycoprotein Requires an Intact Receptor-Binding Domain and Can Be Blocked by GP1-Specific Antibodies

Author

Brinkmann, Constantin, primary, Nehlmeier, Inga, additional, Walendy-Gnirß, Kerstin, additional, Nehls, Julia, additional, González Hernández, Mariana, additional, Hoffmann, Markus, additional, Qiu, Xiangguo, additional, Takada, Ayato, additional, Schindler, Michael, additional, and Pöhlmann, Stefan, additional

Published
2016
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9. Functional Properties and Genetic Relatedness of the Fusion and Hemagglutinin-Neuraminidase Proteins of a Mumps Virus-Like Bat Virus

Author

Krüger, Nadine, primary, Hoffmann, Markus, additional, Drexler, Jan Felix, additional, Müller, Marcel Alexander, additional, Corman, Victor Max, additional, Sauder, Christian, additional, Rubin, Steven, additional, He, Biao, additional, Örvell, Claes, additional, Drosten, Christian, additional, and Herrler, Georg, additional

Published
2015
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11. Surface Glycoproteins of an African Henipavirus Induce Syncytium Formation in a Cell Line Derived from an African Fruit Bat, Hypsignathus monstrosus

Author

Krüger, Nadine, primary, Hoffmann, Markus, additional, Weis, Michael, additional, Drexler, Jan Felix, additional, Müller, Marcel Alexander, additional, Winter, Christine, additional, Corman, Victor Max, additional, Gützkow, Tim, additional, Drosten, Christian, additional, Maisner, Andrea, additional, and Herrler, Georg, additional

Published
2013
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12. Rapid SARS-CoV-2 Adaptation to Available Cellular Proteases.

Author

Zeeshan Chaudhry, M., Eschke, Kathrin, Hoffmann, Markus, Grashoff, Martina, Abassi, Leila, Kim, Yeonsu, Brunotte, Linda, Ludwig, Stephan, Kröger, Andrea, Klawonn, Frank, Pöhlmann, Stefan H., and Cicin-Sain, Luka

Subjects
*SARS-CoV-2 Delta variant, *SARS-CoV-2 Omicron variant, *SARS-CoV-2, *PROTEOLYTIC enzymes, *HEPARAN sulfate
Abstract

Recent emergence of SARS-CoV-1 variants demonstrates the potential of this virus for targeted evolution, despite its overall genomic stability. Here we show the dynamics and the mechanisms behind the rapid adaptation of SARS-CoV-2 to growth in Vero E6 cells. The selective advantage for growth in Vero E6 cells is due to increased cleavage efficiency by cathepsins at the mutated S1/S2 site. S1/S2 site also constitutes a heparan sulfate (HS) binding motif that influenced virus growth in Vero E6 cells, but HS antagonist did not inhibit virus adaptation in these cells. The entry of Vero E6-adapted virus into human cells is defective because the mutated spike variants are poorly processed by furin or TMPRSS2. Minor subpopulation that lack the furin cleavage motif in the spike protein rapidly become dominant upon passaging through Vero E6 cells, but wild type sequences are maintained at low percentage in the virus swarm and mediate a rapid reverse adaptation if the virus is passaged again on TMPRSS21 human cells. Our data show that the spike protein of SARSCoV- 2 can rapidly adapt itself to available proteases and argue for deep sequence surveillance to identify the emergence of novel variants. IMPORTANCE Recently emerging SARS-CoV-2 variants B.1.1.7 (alpha variant), B.1.617.2 (delta variant), and B.1.1.529 (omicron variant) harbor spike mutations and have been linked to increased virus pathogenesis. The emergence of these novel variants highlights coronavirus adaptation and evolution potential, despite the stable consensus genotype of clinical isolates. We show that subdominant variants maintained in the virus population enable the virus to rapidly adapt to selection pressure. Although these adaptations lead to genotype change, the change is not absolute and genomes with original genotype are maintained in the virus swarm. Thus, our results imply that the relative stability of SARS-CoV-2 in numerous independent clinical isolates belies its potential for rapid adaptation to new conditions. [ABSTRACT FROM AUTHOR]

Published
2022
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