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Your search keyword '"Kohei Oishi"' showing total 4 results
Start Over Author "Kohei Oishi" Journal journal of virology
4 results on '"Kohei Oishi"'

1. Archaeal Kink-Turn Binding Protein Mediates Inhibition of Orthomyxovirus Splicing Biology

Author

Kohei Oishi, Daniel Blanco-Melo, Andrew P. Kurland, Jeffrey R. Johnson, and Benjamin R. tenOever

Subjects
Virology, Insect Science, Immunology, Microbiology
Abstract

Here, we demonstrate that a family of proteins from archaea specifically inhibit this splicing biology of all tested members of the Orthomyxoviridae family. We show that this inhibition extends to influenza A virus, influenza B virus, and isavirus genera, while having no significant impact on the mammalian transcriptome or proteome.

Published
2023

2. The Host Response to Influenza A Virus Interferes with SARS-CoV-2 Replication during Coinfection

Author

Kohei Oishi, Shu Horiuchi, Judith M. Minkoff, and Benjamin R. tenOever

Subjects
Mesocricetus, Coinfection, SARS-CoV-2, Immunology, COVID-19, Virus Replication, Microbiology, Cross-Priming, Influenza A Virus, H1N1 Subtype, Virology, Insect Science, Influenza, Human, Animals, Humans, Interferons
Abstract

The human population now has two circulating respiratory RNA viruses with high pandemic potential, namely, SARS-CoV-2 and influenza A virus. As both viruses infect the airways and can result in significant morbidity and mortality, it is imperative that we also understand the consequences of getting coinfected.

Published
2022

3. The Host Response to Influenza A Virus Interferes with SARSCoV-2 Replication during Coinfection.

Author

Kohei Oishi, Shu Horiuchi, Minkoff, Judith M., and tenOever, Benjamin R.

Subjects
*COVID-19, *H1N1 influenza, *SARS-CoV-2, *PANDEMICS, *INFLUENZA viruses, *TYPE I interferons, *INFLUENZA A virus, *MIXED infections
Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza A virus (IAV) represent two highly transmissible airborne pathogens with pandemic capabilities. Although these viruses belong to separate virus families--SARS-CoV-2 is a member of the family Coronaviridae, while IAV is a member of the family Orthomyxoviridae--both have shown zoonotic potential, with significant animal reservoirs in species in close contact with humans. The two viruses are similar in their capacity to infect human airways, and coinfections resulting in significant morbidity and mortality have been documented. Here, we investigate the interaction between SARS-CoV-2 USA-WA1/2020 and influenza H1N1 A/California/04/2009 virus during coinfection. Competition assays in vitro were performed in susceptible cells that were either interferon type I/III (IFN-I/-III) nonresponsive or IFN-I/-III responsive, in addition to an in vivo golden hamster model. We find that SARS-CoV-2 infection does not interfere with IAV biology in vivo, regardless of timing between the infections. In contrast, we observe a significant loss of SARS-CoV-2 replication following IAV infection. The latter phenotype correlates with increased levels of IFN-I/-III and immune priming that interferes with the kinetics of SARS-CoV-2 replication. Together, these data suggest that cocirculation of SARS-CoV-2 and IAV is unlikely to result in increased severity of disease. [ABSTRACT FROM AUTHOR]

Published
2022
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4. Mapping of a Region of the PA-X Protein of Influenza A Virus That Is Important for Its Shutoff Activity

Author

Kohei Oishi, Seiya Yamayoshi, and Yoshihiro Kawaoka

Subjects
Molecular Sequence Data, Immunology, Viral Nonstructural Proteins, medicine.disease_cause, Microbiology, Open Reading Frames, Endonuclease, Virology, Influenza A virus, medicine, Amino Acid Sequence, Luciferases, Gene, Basic amino acids, chemistry.chemical_classification, Messenger RNA, Base Sequence, biology, Host (biology), Sequence Analysis, DNA, Endonucleases, Molecular biology, Protein Structure, Tertiary, Genome Replication and Regulation of Viral Gene Expression, Amino acid, Repressor Proteins, chemistry, Mutagenesis, Insect Science, biology.protein, Function (biology)
Abstract

Influenza A virus PA-X comprises an N-terminal PA endonuclease domain and a C-terminal PA-X-specific domain. PA-X reduces host and viral mRNA accumulation via its endonuclease function. Here, we found that the N-terminal 15 amino acids, particularly six basic amino acids, in the C-terminal PA-X-specific region are important for PA-X shutoff activity. These six basic amino acids enabled a PA deletion mutant to suppress protein expression at a level comparable to that of wild-type PA-X.

Published
2015

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