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Background and Objectives: Radiology technicians have a critical role in protecting patients along with providing high-quality images. The present study assessed radiology students' last semester about the principles of protection, dose level, and cancer risk of ionizing radiation applied for diagnostic imaging. This study was conducted in training hospitals of Ahvaz Jundishapur University of Medical Sciences, Ahvaz City, Iran. Subjects and Methods This cross-sectional descriptive study was performed using a study-designed questionnaire. The sample size was 40, and statistical analysis was performed in SPSS v. 24. Results Only 15% of students correctly answered the question of ionizing radiation stochastic effect. While 70% of students stated the average dose of an abdominal ultrasound correctly, only 2.5% of them determined the average dose of a myocardial nuclear medicine scan correctly. In total, the respondents received 35% of the overall score of knowledge about cancer risk caused by ionizing radiation of imaging examinations. Conclusion The students' knowledge about the principles of radiation protection was moderate, and their knowledge about the level of radiation dose and cancer risk caused by various imaging procedures was insufficient. Theoretical and practical education has a significant role in improving the knowledge and skills of radiology technicians. So, repeating and emphasizing the principles of radiation protection and paying more attention to these topics in the courses and internships of radiology technology students seems necessary. These measures will improve the performance of students and make them prepared to accept their future professional responsibilities. [ABSTRACT FROM AUTHOR]