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Abstract Halal food is an essential thing to be considered by the Muslim community in Indonesia. Halal food must be free from pork, both as a primary ingredient and in the manufacturing process. Halal problems arise when there is a process of mixing meat or lard (adulteration) in halal animal meat for economic purposes. This study aims to identify the authenticity of beef circulating in several traditional markets in Yogyakarta using the Rapid Pork Detection Test and Enzyme-Linked Immunosorbent Assay (ELISA) detection methods. There were 10 samples of beef from several traditional markets in Yogyakarta. The tests carried out were organoleptic tests, Rapid Tests, and ELISA tests. The Rapid Pork Detection Test used is a special Rapid Test for detection of Xema® brand pork produced by PT. Indo Techno Plus. Sampling was carried out from May to June, the markets visited for sampling were the Pingit, Kranggan, Demangan, Ngasem, Godean, Sleman, Gamping, Mangiran and Sentul markets. The organoleptic examination includes odor, color, consistency, and pH. The results of the physical quality test of the meat showed that the beef circulating in the ten markets had a distinctive beef smell, red and pale red, had a chewy consistency that did not break down easily, and had a pH of 5 to 6 except for one sample (P9) from Sentul Market. The results of the Rapid Pork Detection Test showed a negative (-) pork content for all the beef samples. Meanwhile, in the ELISA test, there were three samples with positive results containing pork. The quality of beef circulating in these markets is within the normal range of meat quality and is safe for consumption. Keywords: Pork adulteration; Rapid Pork Detection; ELISA; meat physical quality. Abstrak Makanan halal merupakan suatu hal yang sangat penting untuk diperhatikan oleh masyarakat muslim di Indonesia. Makanan halal harus terbebas dari kandungan babi baik sebagai bahan dasar maupun dalam proses pembuatannya. Permasalahan kehalalan timbul ketika terdapat proses pencampuran daging atau lemak babi (adulterasi) pada daging hewan halal untuk tujuan ekonomis. Penelitian ini bertujuan untuk mengidentifikasi keaslian daging sapi yang beredar di beberapa pasar tradisional di Yogyakarta menggunakan metode deteksi Rapid Pork Detection Test dan Enzyme Linked Immunosorbent Assay (ELISA). Sampel berjumlah sebanyak 10 sampel daging sapi dari beberapa pasar tradisional di Yogyakarta. Uji yang dilakuan adalah uji organoleptik, uji Rapid Test dan uji ELISA. Rapid Pork Detection Test yang digunakan adalah Rapid Test khusus deteksi daging babi merk Xema® produksi PT. Indo Tekhno Plus. Sampling dilakukan pada bulan Mei sampai dengan Juni, pasar yang di kunjungi untuk pengambilan sampel adalah pasar Pingit, Kranggan, Demangan, Ngasem, Godean, Sleman, Gamping, Mangiran dan Sentul. Pemeriksaan organoleptik meliputi bau, warna, konsistensi dan pH. Hasil uji kualitas fisik daging menunjukkan bahwa daging sapi yang beredar di sepuluh pasar tersebut memiliki bau khas daging sapi, berwarna merah dan merah pucat, memiliki konsistensi kenyal tidak mudah terurai, dan memiliki pH 5 sampai dengan 6 kecuali satu sampel (P9) dari Pasar Sentul. Hasil uji Rapid Pork Detection Test menunjukkan hasil negatif (-) kandungan daging babi untuk semua sampel daging sapi tersebut. Sedangkan pada uji ELISA terdapat satu sampel dengan hasil positif mengandung babi. Kualitas daging sapi yang beredar di pasar-pasar tersebut masih dalam kisaran kualitas daging normal dan aman untuk dikonsumsi. Uji cemaran daging babi dapat dilakukan dengan menggunakan metode ELISA karena memiliki tingkat sensitivitas yang lebih tinggi dibandingkan dengan pengujian rapid test Kata kunci: Campuran babi; Rapid Pork Detection; ELISA; Kualitas fisik daging.

Abstract The main obstacle of invasive hormone research of Etawah crossbreed goats (PE) were the sample collection due to the aggressiveness of PE goats. Improper ways of handling the goats would result in stress which leads to bias on the data. This research aims to determine the level of fecal progesterone of PE goats during pregnancy. This research use 5 pregnant and 5 non-pregnant PE goats. Diagnosis of pregnancy was conducted by observing the absence of estrus after breeding. Fecal collection was conducted in week 4, 8, 12, 16, 20, and 2 weeks after parturition but sample collection of non-pregnant goats conducted once. The sample of fecal which has been collected then frozen and extracted using methanol 80% as much as 3 ml. The fecal extract was analyzed using EIA KIT progesterone. The results of measurement of fecal progesterone level were 6,282 ± 950.96 ng/gr of feces (week 4 of pregnancy); 18,391.8 ± 2,584 ng/gr (week 8 of pregnancy); 25,958.4 ± 3,447.1 ng/gr of feces (week 12 of pregnancy); 25,233.4 is ± 3,306 ng/gr of feces (week 16 of pregnancy); 18,238.2 ± 3,069.5 ng/gr of feces (week 20 of pregnancy). The level of fecal progesterone in 2 weeks after giving birth was 516 ± 228.16 ng/gr of feces. Meanwhile, the level of fecal progesterone of non-pregnant PE goats is 254.2± 214.5 ng/gr of feces. The conclusion of the progesterone level of PE goats during pregnancy can be detected using the sample of feces with the highest level in week 12 of pregnancy. Keywords: Feces; Non-invasive; PE Goats; Progesterone Abstrak Kendala utama penelitian hormonal secara invasive pada kambing Peranakan Ettawah (PE) pengambilan sampel pada hewan tersebut karena karakteristik kambing PE yang sangat agresif. Handling yang tidak tepat dapat menjadi stresor yang akan menghasilkan data yang bias. Penelitian ini bertujuan untuk mengukur kadar hormon progesteron feses pada kambing PE selama kebuntingan. Penelitian ini menggunakan 5 ekor kambing PE bunting dan 5 kambing PE tidak bunting. Diagnosa kebuntingan dilakukan dengan pengamatan tidak munculnya estrus setelah pengawinan. Pengambilan feses dilakukan pada minggu ke 4, 8, 12, 16 dan ke 20 dan 2 minggu setelah partus. Pada kambing tidak bunting, pengambilan sampel dilakukan 1 kali. Sampel feses yang telah terkumpul, dikeringbeku dan kemudian diekstraksi menggunakan methanol 80% sebanyak 3 ml untuk selanjutnya dianalisis menggunakan KIT Progesteron (Calbiotech R - USA). Hasil uji pararelism menunjukkan adanya penurunan OD secara paralel seiring dengan besarnya pengenceran sampel. Kadar progesteron feses didapatkan, 6.282 ± 950.96 ng/gr feses (minggu ke 4 kebuntingan); 18.391,8 ± 2584 ng/gr fese (minggu ke 8 kebuntingan); 25.958, 4 ± 3.447,1 ng/gr feses (minggu ke 12 kebuntingan); 25.233,4 ± 3.306 ng/gr feses (minggu ke 16 kebuntingan); 18.238,2 ± 3.069,5 ng/gr feses (minggu ke 20 kebuntingan). Kadar progesteron feses 2 minggu setelah melahirkan adalah 516 ± 228,16 ng/gr feses sedangkan progesteron kambing PE tidak bunting adalah 254,2 ± 214,5 ng/gr feses. Kesimpulan kadar progesteron kambing PE selama kebuntingan dapat dideteksi menggunakan sampel feses dengan kadar tertinggi pada minggu ke 12 kebuntingan. Kata Kunci: Feses; Kambing PE, Non-invasif, Progesteron

Abstract Day Old Chicken is a living commodity that is widely distributed between islands throughout Indonesia. Transport trip within two to 24 hours, depending on the distance of the enclosure to be addressed. During the Day-Old Chicken journey, a variety of stresses result in stress and also end in death. Strain Day-Old Chicken has different endurance. Physiological stress parameters in poultry by calculating the ratio of Heterophil: Lymphocytes (H: L). This study aims to determine the difference in stress between Day-Old Chicken strain Cobb and Java Super passed through. Research carried out using Day-Old Chicken strain Cobb as many as 100 chicken/box and Java Super 100 chicken /box. The transportation process from Yogyakarta airport (loading) to Makassar airport (landing). Furthermore, Day-Old Chicken was taken four animals randomly before and after it was passed for blood smear preparations. Blood smear preparations with Giemza staining to calculate the H: L ratio. Analysis of H: L ratio data as a comparison of body resistance to stress between Day-Old Chicken strain Cobb and Java Super using Two Way Anova with a 95% confidence level. The results showed that there was no interaction (P> 0.05) between location (loading-landing) and Day-Old Chicken strain different from H: L ratio. The difference in the Day-Old Chicken strain affects the H: L ratio (P 0,05) antara lokasi (loading-landing) dan galur Day Old Chicken berbeda terhadap rasio H:L. Perbedaan galur Day Old Chicken mempengaruhi rasio H:L (P < 0,05) dengan nilai rata-rata rasio H:L Day Old Chicken galur Super Jawa lebih tinggi dibandingkan galur Cobb. Kesimpulan bahwa perbedaan galur Day Old Chicken yang mengalami transportasi menggunakan pesawat terbang mempengaruhi ketahanan tubuh terhadap stres. Kata Kunci: Day Old Chicken; Heterofil; Limfosit; Stres; Transportasi.

Abstract The potential of seaweed as a functional food ingredient has not been explored. Several studies, the compotition of seaweed can prevent stress in animals such as sedative mechanisms. The sedative effect was decreased of blood pressure. Pregnancy can be a stressor in animals and cause anemia physiologically. This study aims to determine the effect of seaweed extract on the profile hemoglobin and erythrocytes of pregnant mice. In this study used 10 pregnant rats divided into 2 groups, group 1 (control) and group II (treatment with 450 mg/BW extract Sargassum sp). Calculation of erythrocytes, hemoglobin, and thrombocyte measurements were carried out in day 0, 7, and 14. The results showed that there was an increased thrombocyte count in the treatment group even though in both groups there was a decreased the number of erythrocytes and hemoglobin. The results of statistical analysis showed a significant difference (p