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DNA extraction is the first step in obtaining high-concentration and high-purity DNA that can use in the subsequent steps. Because the leaf structure is challenging and contains several secondary metabolites that can affect DNA extraction results, DNA extraction on dry leaves of Dipterocarpaceae is considered problematic. This research aims to find a suitable method for extracting DNA from the preserved Dipterocarpaceae leaves. Preserved leaves of Dipterocarpaceae in silica gel will dry up, making them tough to destroy. Perform DNA extraction is generally using CTAB buffers or extraction kits. According to the results of this study, extraction with modified CTAB buffer result in DNA with higher concentration and purity values than DNA extracted with the kit. DNA isolated from the modified CTAB can be used as a template in the PCR process to amplify the matK gene.

Heavy metal contaminants like mercury is a serious problem to human, animals, and some plants’ health. Phytoremediation is an alternative technique, which can remediate the contaminants from soil using a hyperaccumulator plant. The goal of this research was to study anatomical changes of plant main organs (roots, stems, and leaves) of Ipomoea reptans which are assumed as a hyperaccumulator plant that grows in mercury contaminant. The mercury concentration of the growth medium was 0, 61.871, 92.258, and 107.046 ppb. Ipomoea reptans were harvested after 27 days. The anatomical changes of the plant’s main organs were observed by preparing the cross-section of roots, stems, and leaves of I. reptans. The result showed that mercury treatment has caused anatomical damage at the xylem vessel of the root, and decreased bulliform cell size. The anatomical damage was found only in the root of I. reptans. The mercury concentration in media decrease to 0 ppb (P0), 50,420 ppb (P1), 58,583 (P2), and 96,120 (P3).