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This article analyzes the macular choroidal thickness in normal Taiwanese eyes using enhanced depth imaging optical coherence tomography (EDI OCT). The study includes 295 eyes of 295 normal Taiwanese subjects and examines the relationships between choroidal thickness and various clinical parameters. The results show that age and spherical equivalent refractive error (SERE) have a significant correlation with macular choroidal thickness, while gender and axial length (AL) do not. The study also finds that choroidal thickness decreases with age and is thinner in individuals with myopia. These findings highlight the importance of identifying clinical risk factors for macular choroidal thinning in order to preserve eye vision effectively. [Extracted from the article]

Fluorescein angiography showed disc edema with dye leakage (OD; Figure 1C) and a single point of hyperfluorescence with focal area of retinal pigment epithelium (RPE) decompensation over the temporal superior vessel arcade (OS; Figure 1D). At the visit of six-week follow-up, the disc edema (OD) and the serous detachment (OS) were both reduced (Figure 1E,F) with best corrected visual acuity (BCVA) restored to 20/20 (OD) and 20/25 (OS). (A) Initial fundus photography of the right eye shows optic disc edema. [Extracted from the article]

Extracellular matrix (ECM) plays an active and complex role in regulating cellular behaviors, including proliferation and adhesion. This study aimed at delineating the adhesion-induced signaling profiles in cultured human retinal pigment epithelium (RPE) cells and investigating the antiadhesion effect of antiproliferative drugs in this context. RPE R-50 cells grown on various ECM molecules, such as type I and IV collagens, fibronectin, and laminin, were used for adhesion assay and for examining the phosphorylation profiles of signaling mediators including Akt, extracellular signal-regulated kinase (ERK) 1/2, and integrin-linked kinase (ILK) using Western blotting. The cells receiving antiproliferative drug treatment at subtoxic doses were used to evaluate their antiadhesive and suppressive effects on kinase activities. ECM coating enhanced adhesion and spreading of RPE cells significantly. The cellular attachment onto ECM-coated surfaces differentially induced Akt, ERK1/2, and ILK phosphorylation, and concomitantly increased p53 phosphorylation and cyclin D1 expression, but decreased Bcl-2/Bax ratios. Treatment with antiproliferative agents, including 5-fluorouracil, mitomycin C, and daunomycin, at subtoxic doses suppressed the ability of RPE cells to adhere to ECM substratum significantly. This suppression was in part mediated through reduction of integrin β1 and β3 expressions and interfering Akt-ILK signaling activity. Mechanistically, blockade of PI3K/Akt signaling resulted in the suppressed adhesion of RPE cells to ECM. These findings support the hypothesis that, in addition to their antimitogenic effect, antiproliferative agents also exhibit suppressive effect on the adhesiveness of cultured RPE cells. Moreover, inhibitors of the PI3K/Akt signaling mediator can potentially be used as therapeutic agents for proliferative vitreoretinopathy., (Copyright © 2013. Published by Elsevier B.V.)