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Purpose: This study aims to add to the current understanding of mediation and moderation processes through which employee career orientation (CO) is linked with organizational citizenship behavior (OCB). Design/methodology/approach: Data were collected from 214 employees working in Delhi and NCR of India. Structural equation modeling (SEM) was used for testing moderated mediation and establishing linkages between CO, CMP and OCB. Drawing on the social exchange theory, our model posits that the effect of CO on the outcome variable OCB is mediated by career management practices (CMPs) and the CMP-mediated relationship between the two is moderated by the gender of employees. Findings: Overall, data from 214 employees from service organizations in India support the model. This suggests that the enactment of OCB as a consequence of CO and OCB is largely dependent on the gender of employees. The relationship was observed in such a way that for women employees, CMP will have a stronger influence on the CO-OCB relationship. According to bootstrap results, upon the addition of CMP as a mediator, the main effect of CO on OCB among male employees was significant but dropped from the Beta value of 0.281 to 0.196. However, adding CMP as a mediator among women employees caused the CO-OCB relationship to become insignificant (Beta = 0.124; LLCI = 0.415; ULCI = −0.127; p = 0.420), highlighting that CMP would have a more substantial influence on the CO-OCB relationship. Originality/value: This study explains the mediational role of CMP in the relationship between CO and OCB (explaining how the employees with new CO can trigger the role of CMP, and consequently, CMP can help them enact OCB) and how the gender of employees moderate the mediated impact of CMP in the relationship between CO and OCB (explaining how the mediated relationship varies across genders). The novelty of the study lies in exploring such a relationship that has not been studied so far. [ABSTRACT FROM AUTHOR]