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We introduce non-aqueous continuous-flow electrophoresis (NACFE) in which the electrolyte is a solution of an organic salt in an aprotic organic solvent. NACFE can maintain steady-state separation of multiple hydrophobic organic species into individual molecular streams. It is a potential separation complement for continuous-flow organic synthesis. This proof-of-concept work will serve as a justification for efforts towards making NACFE a practical tool in flow chemistry.

We present an image processing and analysis system to facilitate detailed performance analysis of free flow electrophoresis (FFE) chips. It consists of a cost-effective self-built imaging setup and a comprehensive customizable software suite. Both components were designed modularly to be accessible, adaptable, versatile, and automatable. The system provides tools for i) automated identification of chip features (e.g. separation zone and flow markers), ii) extraction and analysis of stream trajectories, and iii) evaluation of flow profiles and separation quality (e.g. determination of resolution). Equipped with these tools, the presented image processing and analysis system will enable faster development of FFE chips and applications. It will also serve as a robust detector for fluorescence-based analytical applications of FFE.