1. Spontaneous and experimental infections of Xenopus laevis with Chlamydia psittaci.
- Author
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Newcomer CE, Anver MR, Simmons JL, Wilcke BW Jr, and Nace GW
- Subjects
- Animals, Chlamydia trachomatis ultrastructure, Chlamydophila psittaci isolation & purification, Kidney pathology, Liver pathology, Lung pathology, Microscopy, Electron, Myocardium pathology, Psittacosis diagnosis, Psittacosis pathology, Spleen pathology, Psittacosis veterinary, Xenopus laevis
- Abstract
Chlamydial infection was suspected when widespread pyogranulomatous inflammation and large basophilic intracytoplasmic inclusion bodies were evident histopathologically in African clawed frogs (Xenopus laevis) dying of a spontaneous disease of high morbidity and mortality. Organism morphology was determined by electron microscopy of infected hepatic sinusoidal lining cells, and it was characteristic of the unique developmental cycle of a chlamydial agent. Isolation and speciation of the organism was achieved in a McCoy cell culture system. The infected cells were inoculated into disease-free frogs reproducing the disease.
- Published
- 1982