1. Absolute CD4 count and percentage values among Libyan patients with HIV by single-platform flow cytometry.
- Author
-
Lamami, Yosra, Abulayha, Abdulmunem M, Altabal, Salah, Elbasir, Mohamed, Elbnnani, Abdulrhman S, Aghil, Laila, Ebrahim, Fawzi, and Elzagheid, Adam
- Subjects
- *
FLOW cytometry , *REFERENCE values , *T cells , *DATA analysis , *BLOOD testing , *HIV-positive persons , *CD4 lymphocyte count , *HIV infections , *TREATMENT effectiveness , *DESCRIPTIVE statistics , *HOSPITALS , *MANN Whitney U Test , *ANTIVIRAL agents , *MONOCLONAL antibodies , *ONE-way analysis of variance , *STATISTICS , *PATIENT monitoring , *COMPARATIVE studies , *DATA analysis software , *DISEASE progression , *SENSITIVITY & specificity (Statistics) , *NONPARAMETRIC statistics - Abstract
Background Single-platform flow cytometry technology together with CD45-gating is becoming the method of choice for absolute CD4 T cell enumeration. Immunological assessment of HIV patients by monitoring CD4 can provide valuable information on antiviral treatment response and disease progression. Methods A total of 97 HIV-positive individuals were recruited from 2 hospitals in Tripoli, Libya, and 14 healthy blood donors. The HIV-infected individuals were classified by CD4+ count into HIV-positive (>200 cells/µL) or AIDS (≤200 cells/µL) groups. CD4+ and CD8+ cell counts were determined and compared among the groups and with similar published data. Results The mean ± SD CD4+ cell counts were 1106 ± 442.8 cells/µL in healthy individuals, 460 ± 219.7 cells/µL in the HIV-positive group, and 78 ± 64.3 cells/µL in the AIDS group. The mean ± SD CD4+/CD8+ ratio was 1.6 ± 0.58, 0.4 ± 0.22, and 0.1 ± 0.1, respectively. CD4+ counts in Libyan healthy adults might be higher than those reported in several studies in other regions, whereas CD4+ counts in Libyan AIDS patients seem lower. Conclusion Reference values for T lymphocyte counts in Libyan healthy individuals should be investigated more extensively, and the reasons why Libyan AIDS patients seem to have such lower CD4+ counts should be examined. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF