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Surface runoff is a major component of the hydrological cycle, and it is essential for supporting the ecosystem services provided by grassland and forest ecosystems. It is of practical importance to understand the mechanisms and the dynamic processes of runoff in a river's basin, and in this study, we focused on the restored montane Pailugou Basin in the Qilian Mountains, Gansu Province, China, since its water status is extremely important for the large arid area and local economies therein. Our purpose was to determine the annual variation in the surface runoff in the Pailugou Basin because it is important to understand the influence of climate fluctuations on surface water resources and the economy of the basin. In addition, little is known about the annual variations in precipitation and runoff in this region of the world. Daily atmospheric precipitation, air temperature and runoff data from 2000 to 2019 were analyzed by the calculation of the uneven annual distribution of surface runoff, the calculation of the complete adjustment coefficient, and the vector accumulation expressed by the concentration degree. We also used the cumulative anomaly approach to determine the interannual variation trend of runoff, while the change trend was quantified by the sliding average method. Finally, we used the Mann–Kendall mutation test method and regression analysis to establish the time-series trend for precipitation and runoff and to determine the period of abrupt runoff changes. The results indicated concentrated and positive distributions of surface runoff on an annual basis, with a small degree of dispersion, and an explicit concentration of extreme flows. The relative variation ranges exhibited a decreasing trend, and the distribution of the surface runoff gradually was uniform over the year. The runoff was highest from July to September (85% of the annual total). We also determined that annual surface runoff in the basin fluctuated over the 20-year period but showed an overall increasing trend, increasing by 3.94 × 105 m3, with an average increase rate of 0.42 × 105 m3 every ten years. From 2005 to 2014, the annual runoff and the proportion of runoff in the flood season (July to September) to the annual runoff fluctuated greatly. The correlation between the runoff and precipitation was significant (r = 0.839, p < 0.05), whereas the correlation between air temperature and surface runoff was low (r = 0.421, p < 0.05). [ABSTRACT FROM AUTHOR]