1. An Anticaffeine Antibody-Oligonucleotide Conjugate for DNA-Directed Immobilization in Environmental Immunoarrays
- Author
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Rudolf J. Schneider, Cinthya Véliz Montes, Ana Margarida Carvalho, and Annemieke Madder
- Subjects
medicine.drug_class ,Immobilized Nucleic Acids ,02 engineering and technology ,Computational biology ,010402 general chemistry ,Monoclonal antibody ,01 natural sciences ,Proof of Concept Study ,chemistry.chemical_compound ,Caffeine ,Electrochemistry ,medicine ,General Materials Science ,Routine analysis ,Spectroscopy ,Immunoassay ,Complex matrix ,biology ,Oligonucleotide ,Antibodies, Monoclonal ,Nucleic Acid Hybridization ,Surfaces and Interfaces ,DNA ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,0104 chemical sciences ,High-Throughput Screening Assays ,Specific antibody ,chemistry ,Oligodeoxyribonucleotides ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,biology.protein ,Central Nervous System Stimulants ,Electrophoresis, Polyacrylamide Gel ,Antibody ,0210 nano-technology ,DNA Probes ,Water Pollutants, Chemical ,Conjugate - Abstract
The development of fast and cheap high-throughput platforms for the detection of environmental contaminants is of particular importance to understand the human-related impact on the environment. The application of DNA-directed immobilization (DDI) of IgG molecules is currently limited to the clinical diagnostics scenario, possibly because of the high costs of production of such addressable platforms. We here describe the efficient and specific hybridization of an antibody-oligonucleotide conjugate to a short 12-mer capture probe. The specific antibody used is a monoclonal antibody against caffeine, a stimulant and important anthropogenic marker. With this work, we hope to contribute to broadening the application potential of DDI to environmental markers in order to develop cheaper and more stable high-throughput screening platforms for standard routine analysis of pollutants in a variety of complex matrices.
- Published
- 2018