1. Immobilization of Enzymes on Flexible Tubing Surfaces for Continuous Bioassays.
- Author
-
Jannat M and Yang KL
- Subjects
- Amines chemistry, Animals, Caseins metabolism, Cattle, Dextrans chemistry, Enzyme Stability, Hydrogen Peroxide chemistry, Hydrolysis, Silanes chemistry, Surface Properties, Biological Assay, Catalase chemistry, Catalase metabolism, Enzymes, Immobilized chemistry, Enzymes, Immobilized metabolism, Mechanical Phenomena
- Abstract
Immobilized enzymes can be used to catalyze biochemical reactions in a batch process, however, it is more difficult to use them in a continuous process. Herein, we develop an enzyme immobilization technique for flexible tubing surfaces, which can be used to catalyze biochemical reactions in a continuous process. In this technique, the tubing is first treated with (3-aminopropyl)triethoxysilane at 50 °C and baked at 100 °C in vacuum to form a network of reactive amine functional group on the inner tubing surface. Subsequently, dextran polyaldehyde, a polymeric cross-linker, is used to immobilize crude protease extract and catalase for hydrolyzing casein and degrading H
2 O2 , respectively, in a continuous process. The immobilized proteases are highly stable even after a long-term storage at 4 °C. After 12 weeks of storage, 90% of the original protease activity can be preserved. Meanwhile, the immobilized catalase is able to degrade 0.1% H2 O2 solution flowing at 5 μL/min. The immobilization technique is potentially useful for bioassays and industrial wastewater treatments when continuous processes are preferred.- Published
- 2018
- Full Text
- View/download PDF