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Introduction: This study evaluates the assessment protocol that allows the correlation between the development of mesothelioma to a specific exposure, with particular focus on investigations with Scanning Electron Microscope with Energy Dispersion Spectroscopy., Methods: This retrospective study includes 80 subjects who died from mesothelioma in the period 2001-2019. A judicial autopsy was performed for each case to confirm cause of death and correlate the disease with specific asbestos exposure. In 28 cases investigations were carried out to determine the pulmonary load of the asbestos fibres and corpuscles in the lung tissue through microscopic investigations, in order to confirm the suspicion of occupational exposure., Results: Our data agree with the scientific literature reported, but it is interesting to underline how the present study uses a different systematic approach than others, which are mainly based on epidemiological and environmental studies without considering the lung content of fibres and corpuscles., Conclusion: It would be desirable that the use of the microscopic analysis was introduced in the evaluation protocol: it should always be carried out if the suspicion of asbestos-related disease is raised and not only as a possible integration to the less expensive anamnestic evaluation, even more so if the work or personal history should be suggestive of exposure to asbestos fibres., (Copyright © 2022 Elsevier B.V. All rights reserved.)

DNA profiling can identify an individual from a sample of biological material but it does not reveal what body fluid or tissue source the DNA profile originated from. In many cases it is important to know from what body fluid or tissue the DNA profile originated in order to provide crucial information necessary to the investigation, especially in cases where the victims are not able to give information about the dynamics of the event. For this purpose messenger RNA (mRNA) analysis has been shown to be a suitable method for the identification of body fluids, resulting in a trend to overcome the conventional approaches. Here we present the first report about case regarding a three-year-old child supposedly victim of a sexual assault with digital penetration. Thanks to the use of the combined DNA profiling and RNA analysis it was possible to demonstrate the sexual assault suffered by the victim., (Copyright © 2020 Elsevier B.V. All rights reserved.)

One hundred twenty-five unrelated individuals (69 females and 56 males) from Sassari (Northern Sardinia) and Orgosolo (Central Sardinia) were typed for 15 STRs loci. The 56 males were typed for 12 Y chromosome STRs loci too. Frequency distribution is described.

One hundred and thirty unrelated individuals (80 females and 50 males) from Brescia (northern Italy) were typed for DXS8378, DXS7132, HPRTB and DXS7423 loci. Allele, genotype and haplotype frequencies were calculated. A comparison between our population data and others from Caucasians and Asians populations was performed.